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Literature summary extracted from

  • Shafqat, N.; Elleby, B.; Svensson, S.; Shafqat, J.; Jornvall, H.; Abrahmsen, L.; Oppermann, U.
    Comparative enzymology of 11beta -hydroxysteroid dehydrogenase type 1 from glucocorticoid resistant (guinea pig) versus sensitive (human) species (2003), J. Biol. Chem., 278, 2030-2035.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.146 expression of 11beta-HSD1 in Pichia pastoris, COS cells and Escherichia coli Cavia porcellus
1.1.1.146 expression of 11beta-HSD1 in Pichia pastoris, COS cells and Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.146 0.00028
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1 Cavia porcellus
1.1.1.146 0.0003
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 measured in intact COS cells transfected with 11beta-HSD1 Homo sapiens
1.1.1.146 0.0006
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Cavia porcellus
1.1.1.146 0.0008
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Homo sapiens
1.1.1.146 0.002
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris Cavia porcellus
1.1.1.146 0.002
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Pichia pastoris Homo sapiens
1.1.1.146 0.003
-
 cortisol 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Cavia porcellus
1.1.1.146 0.003
-
 cortisol 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Homo sapiens
1.1.1.146 0.008
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1 Cavia porcellus
1.1.1.146 0.009
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 measured in extracts of COS cells transfected with 11beta-HSD1 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.146 cortisone + NADPH + H+ Cavia porcellus
-
 cortisol + NADP+
-
 r
1.1.1.146 cortisone + NADPH + H+ Homo sapiens
-
 cortisol + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.146 Cavia porcellus
-
-
-
1.1.1.146 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.146 recombinant His-tagged-11beta-HSD1 expressed in Escherichia coli Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.146 kidney subcapsular areas of adrenal gland Cavia porcellus
-
1.1.1.146 liver central vein Cavia porcellus
-
1.1.1.146 liver central vein Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.146 cortisone + NADPH + H+
-
 Cavia porcellus cortisol + NADP+
-
 r
1.1.1.146 cortisone + NADPH + H+
-
 Homo sapiens cortisol + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.146 11beta-HSD1
-
 Cavia porcellus
1.1.1.146 11beta-HSD1
-
 Homo sapiens
1.1.1.146 11beta-hydroxysteroid dehydrogenase type 1
-
 Cavia porcellus
1.1.1.146 11beta-hydroxysteroid dehydrogenase type 1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.146 0.04
-
 cortisol 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Cavia porcellus
1.1.1.146 0.19
-
 cortisol 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Homo sapiens
1.1.1.146 0.3
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Cavia porcellus
1.1.1.146 0.4
-
 cortisone 37°C, pH 7.4, recombinant 11beta-HSD1 expressed in Escherichia coli Homo sapiens