EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.3.7 | DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli | Arthrobacter sp. |
3.5.3.10 | DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli | Arthrobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.7 | 1,10-phenanthroline | inactivation due to removal of bound Zn2+, reversible by Zn2+ and Co2+, and partially by Mn2+ | Arthrobacter sp. | |
3.5.3.7 | 5-Aminovalerate | competitive inhibition | Arthrobacter sp. | |
3.5.3.7 | Mercaptoacetate | strong mixed-type inhibitor | Arthrobacter sp. | |
3.5.3.7 | additional information | inhibition by thiol carboxylate, metal binding sequence | Arthrobacter sp. | |
3.5.3.10 | 1,10-phenanthroline | inactivation due to removal of bound Zn2+, reversible by Zn2+ and Co2+, and partially by Mn2+ | Arthrobacter sp. | |
3.5.3.10 | 5-Aminovalerate | competitive inhibition | Arthrobacter sp. | |
3.5.3.10 | Mercaptoacetate | strong mixed-type inhibitor | Arthrobacter sp. | |
3.5.3.10 | additional information | inhibition by thiol carboxylate, metal binding sequence | Arthrobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.7 | additional information | - |
additional information | - |
Arthrobacter sp. | |
3.5.3.10 | additional information | - |
additional information | - |
Arthrobacter sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.3.7 | soluble | - |
Arthrobacter sp. | - |
- |
3.5.3.10 | soluble | - |
Arthrobacter sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.7 | Co2+ | can substitute for Zn2+ | Arthrobacter sp. | |
3.5.3.7 | Mn2+ | can partially substitute for Zn2+ | Arthrobacter sp. | |
3.5.3.7 | Zn2+ | 1 g-atom bound to 1 mol of subunit, dependent on, removal causes loss of activity | Arthrobacter sp. | |
3.5.3.10 | Co2+ | can substitute for Zn2+ | Arthrobacter sp. | |
3.5.3.10 | Mn2+ | can partially substitute for Zn2+ | Arthrobacter sp. | |
3.5.3.10 | Zn2+ | 1 g-atom bound to 1 mol of subunit, dependent on, removal causes loss of activity | Arthrobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.3.7 | 40000 | - |
6 * 40000, SDS-PAGE | Arthrobacter sp. |
3.5.3.7 | 232000 | - |
gel filtration | Arthrobacter sp. |
3.5.3.10 | 40000 | - |
6 * 40000, SDS-PAGE | Arthrobacter sp. |
3.5.3.10 | 232000 | - |
gel filtration | Arthrobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.3.7 | Arthrobacter sp. | Q8KZT5 | strain KUJ8602 | - |
3.5.3.7 | Arthrobacter sp. KUJ 8602 | Q8KZT5 | strain KUJ8602 | - |
3.5.3.10 | Arthrobacter sp. | Q8KZT5 | strain KUJ8602 | - |
3.5.3.10 | Arthrobacter sp. KUJ 8602 | Q8KZT5 | strain KUJ8602 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.3.7 | 113fold | Arthrobacter sp. |
3.5.3.10 | 113fold | Arthrobacter sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.3.7 | 4-guanidinobutanoate + H2O = 4-aminobutanoate + urea | enzyme is identical with D-arginase, EC 3.5.3.10, substrate guanidino group binding sequence | Arthrobacter sp. | |
3.5.3.10 | D-arginine + H2O = D-ornithine + urea | enzyme is identical with guanidinobutyrase, EC 3.5.3.7, substrate guanidino group binding sequence | Arthrobacter sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.3.7 | culture-condition:L-arginine-grown cell | - |
Arthrobacter sp. | - |
3.5.3.7 | additional information | the cells can use D-arginine as sole carbon and nitrogen source | Arthrobacter sp. | - |
3.5.3.10 | culture-condition:L-arginine-grown cell | - |
Arthrobacter sp. | - |
3.5.3.10 | additional information | the cells can use D-arginine as sole carbon and nitrogen source | Arthrobacter sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.3.7 | 8.71 | - |
purified enzyme | Arthrobacter sp. |
3.5.3.10 | 8.71 | - |
purified enzyme | Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.7 | 3-guanidinopropionate + H2O | - |
Arthrobacter sp. | 3-aminopropanoate + urea | - |
? | |
3.5.3.7 | 4-Guanidinobutyrate + H2O | preferred substrate | Arthrobacter sp. | 4-Aminobutyrate + urea | - |
? | |
3.5.3.7 | 4-Guanidinobutyrate + H2O | preferred substrate | Arthrobacter sp. KUJ 8602 | 4-Aminobutyrate + urea | - |
? | |
3.5.3.7 | D-arginine + H2O | - |
Arthrobacter sp. | D-ornithine + urea | - |
? | |
3.5.3.7 | L-arginine + H2O | - |
Arthrobacter sp. | L-ornithine + urea | - |
? | |
3.5.3.10 | 3-guanidinopropionate + H2O | - |
Arthrobacter sp. | 3-aminopropanoate + urea | - |
? | |
3.5.3.10 | 3-guanidinopropionate + H2O | - |
Arthrobacter sp. KUJ 8602 | 3-aminopropanoate + urea | - |
? | |
3.5.3.10 | 4-Guanidinobutyrate + H2O | preferred substrate | Arthrobacter sp. | 4-Aminobutyrate + urea | - |
? | |
3.5.3.10 | 4-Guanidinobutyrate + H2O | preferred substrate | Arthrobacter sp. KUJ 8602 | 4-Aminobutyrate + urea | - |
? | |
3.5.3.10 | D-arginine + H2O | - |
Arthrobacter sp. | D-ornithine + urea | - |
? | |
3.5.3.10 | D-arginine + H2O | - |
Arthrobacter sp. KUJ 8602 | D-ornithine + urea | - |
? | |
3.5.3.10 | L-arginine + H2O | - |
Arthrobacter sp. | L-ornithine + urea | - |
? | |
3.5.3.10 | L-arginine + H2O | - |
Arthrobacter sp. KUJ 8602 | L-ornithine + urea | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.3.7 | hexamer | 6 * 40000, SDS-PAGE | Arthrobacter sp. |
3.5.3.10 | hexamer | 6 * 40000, SDS-PAGE | Arthrobacter sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.3.7 | 4-guanidinobutyrate amidinobutyrase | - |
Arthrobacter sp. |
3.5.3.7 | GBase | - |
Arthrobacter sp. |
3.5.3.7 | More | enzyme is identical with D-arginase, EC 3.5.3.10 | Arthrobacter sp. |
3.5.3.7 | Zn2+-guanidinobutyrase | - |
Arthrobacter sp. |
3.5.3.10 | More | enzyme is identical with guanidinobutyrase, EC 3.5.3.7 | Arthrobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.7 | 37 | - |
assay at | Arthrobacter sp. |
3.5.3.10 | 37 | - |
assay at | Arthrobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.7 | 9 | - |
with substrate 4-guanidinobutyrate | Arthrobacter sp. |
3.5.3.7 | 9.5 | - |
with substrate D-arginine | Arthrobacter sp. |
3.5.3.10 | 9 | - |
with substrate 4-guanidinobutyrate | Arthrobacter sp. |
3.5.3.10 | 9.5 | - |
with substrate D-arginine | Arthrobacter sp. |