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Literature summary extracted from

  • Fournand, D.; Arnaud, A.
    Aliphatic and enantioselective amidases: from hydrolysis to acyl transfer activity (2001), J. Appl. Microbiol., 91, 381-393.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.1
-
 Benzamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 0.1
-
 Isobutyramide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 0.1
-
 butyramide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 0.3
-
 nicotinamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 0.3
-
 hexanamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 1
-
 Propionamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 1.3
-
 L-Leucinamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 2
-
 L-prolinamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 3
-
 Acrylamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 5.4
-
 L-Methioninamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 12
-
 L-alaninamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 16
-
 L-threoninamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3.5.1.4 70
-
 acetamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Cyberlindnera jadinii
-
-
-
3.5.1.4 Mycobacterium avium
-
-
-
3.5.1.4 Rhodococcus sp.
-
-
-
3.5.1.4 Rhodococcus sp. R312
-
-
-
3.5.1.4 Sus scrofa
-
-
-
3.5.1.4 Torula cremoris
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetamide + H2O
-
 Cyberlindnera jadinii acetate + NH3
-
 ?
3.5.1.4 acetamide + H2O
-
 Rhodococcus sp. acetate + NH3
-
 ?
3.5.1.4 acetamide + H2O
-
 Rhodococcus sp. R312 acetate + NH3
-
 ?
3.5.1.4 acetamide + hydroxylamine Ping Pong Bi Bi type mechanism Rhodococcus sp. acetylhydroxamic acid + NH3
-
 ?
3.5.1.4 acetamide + hydroxylamine Ping Pong Bi Bi type mechanism Rhodococcus sp. R312 acetylhydroxamic acid + NH3
-
 ?
3.5.1.4 acrylamide + H2O
-
 Cyberlindnera jadinii acrylate + NH3
-
 ?
3.5.1.4 acrylamide + hydroxylamine
-
 Rhodococcus sp. acrylhydroxamic acid + NH3
-
 ?
3.5.1.4 acrylamide + hydroxylamine
-
 Rhodococcus sp. R312 acrylhydroxamic acid + NH3
-
 ?
3.5.1.4 arachidonoyl-ethanolamine + H2O hydrolysis of anandamide Sus scrofa arachidonic acid + ethanolamine
-
 ?
3.5.1.4 benzamide + hydroxylamine
-
 Rhodococcus sp. benzoylhydroxamic acid + NH3
-
 ?
3.5.1.4 benzamide + hydroxylamine
-
 Rhodococcus sp. R312 benzoylhydroxamic acid + NH3
-
 ?
3.5.1.4 butyramide + H2O
-
 Cyberlindnera jadinii butyrate + NH3
-
 ?
3.5.1.4 butyramide + hydroxylamine
-
 Rhodococcus sp. butyrylhydroxamic acid + NH3
-
 ?
3.5.1.4 butyramide + hydroxylamine
-
 Rhodococcus sp. R312 butyrylhydroxamic acid + NH3
-
 ?
3.5.1.4 hexanamide + H2O
-
 Cyberlindnera jadinii hexanoate + NH3
-
 ?
3.5.1.4 hexanamide + hydroxylamine
-
 Rhodococcus sp. hexanoylhydroxamic acid + NH3
-
 ?
3.5.1.4 isobutyramide + hydroxylamine
-
 Rhodococcus sp. isobutyrylhydroxamic acid + NH3
-
 ?
3.5.1.4 L-alaninamide + hydroxylamine
-
 Rhodococcus sp. L-alaninoylhydroxamic acid + NH3
-
 ?
3.5.1.4 L-leucinamide + hydroxylamine
-
 Rhodococcus sp. L-leucinoylhydroxamic acid + NH3
-
 ?
3.5.1.4 L-methioninamide + hydroxylamine
-
 Rhodococcus sp. L-methioninoylhydroxamic acid + NH3
-
 ?
3.5.1.4 L-prolinamide + hydroxylamine
-
 Rhodococcus sp. L-prolinoylhydroxamic acid + NH3
-
 ?
3.5.1.4 L-threoninamide + hydroxylamine
-
 Rhodococcus sp. L-threoninoylhydroxamic acid + NH3
-
 ?
3.5.1.4 additional information other fatty acid ethanolamides may act as substrate Sus scrofa ?
-
 ?
3.5.1.4 nicotinamide + H2O absolute specificity Mycobacterium avium nicotinic acid + NH3
-
 ?
3.5.1.4 nicotinamide + H2O absolute specificity Torula cremoris nicotinic acid + NH3
-
 ?
3.5.1.4 nicotinamide + hydroxylamine
-
 Rhodococcus sp. nicotinoylhydroxamic acid + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Cyberlindnera jadinii propionate + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Rhodococcus sp. propionate + NH3
-
 ?
3.5.1.4 propionamide + hydroxylamine
-
 Rhodococcus sp. propionylhydroxamic acid + NH3
-
 ?
3.5.1.4 short-chain amides + hydrazine
-
 Rhodococcus sp. acid hydrazides + NH3
-
 ?