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Literature summary extracted from

  • Mazitsos, C.F.; Rigden, D.J.; Tsoungas, P.G.; Clonis, Y.D.
    Galactosyl-mimodye ligands for Pseudomonas fluorescens beta-galactose dehydrogenase (2002), Eur. J. Biochem., 269, 5391-5405.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.48 biotechnology design and synthesis by protein molecular modelling and ligand docking of several chimeric mimodye-ligands comprising a NAD-pseudomimetic moiety of anthraquinone diaminobenzosulfonic acid and a galactosyl-mimetic moiety of 2-amino-2-deoxygalactose or shikimic acid for usage as tailored ligands in selective affinity chromatography during enzyme purification/production, immobilization on a gel resin, overview Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.48 anthraquinone-diamino-benzosulfonyl-dichlorotriazine i.e. Vilmafix Blue A-R, and other derivatives of longer side chain length, inactivation, overview Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.48 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.48 by affinity chromatography on chimeric mimodye-ligand gel resins, method evaluation Pseudomonas fluorescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.48 commercial preparation recombinant, purified Pseudomonas fluorescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.48 D-galactopyranose + NAD(P)+
-
 Pseudomonas fluorescens D-galactono-1,5-lactone + NAD(P)H
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.48 More structure modelling and computational analysis Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.48 beta-galactose dehydrogenase
-
 Pseudomonas fluorescens
1.1.1.48 D-galactose: NAD+ 1-oxidoreductase
-
 Pseudomonas fluorescens
1.1.1.48 GADH
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.48 25
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.48 8.5
-
 assay at Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.48 NAD+ preferred cofactor Pseudomonas fluorescens
1.1.1.48 NADP+
-
 Pseudomonas fluorescens