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Literature summary extracted from

  • Herrero, A.B.; Uccelletti, D.; Hirschberg, C.B.; Dominguez, A.; Abeijon, C.
    The Golgi GDPase of the fungal pathogen Candida albicans affects morphogenesis, glycosylation, and cell wall properties (2002), Eukaryot. Cell, 1, 420-431.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.42 DNA and amino acid sequence determination and analysis, functional complementation of a Saccharomyces GDPase null mutant Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.42 additional information disruption of gene gda1 leads to a mutant with 90% reduced GDP hydrolysis activity compared to the wild-type, the mutant also shows severely impaired O-mannosylation and reduced cell wall phosphate content and other phenotypes, but the formation of hyphae is uneffected, overview Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.42 Golgi apparatus
-
 Candida albicans 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.42 Ca2+ stimulates Candida albicans
3.6.1.42 Mn2+ stimulates, preferred metal ion Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.42 GDP + H2O Candida albicans GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen GMP + phosphate
-
 ?
3.6.1.42 additional information Candida albicans physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.42 Candida albicans Q8TGH6 gene gda1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.42 additional information
-
-
 Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.42 ADP + H2O
-
 Candida albicans AMP + phosphate
-
 ?
3.6.1.42 GDP + H2O preferred substrate Candida albicans GMP + phosphate
-
 ?
3.6.1.42 GDP + H2O GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen Candida albicans GMP + phosphate
-
 ?
3.6.1.42 additional information physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview Candida albicans ?
-
 ?
3.6.1.42 UDP + H2O
-
 Candida albicans UMP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.42 dimer enzyme contains a unique sequence in the stem region predicted with high probability to adopt a coil-coil conformation that favors dimerization Candida albicans

Synonyms

EC Number Synonyms Comment Organism
3.6.1.42 Gdap1
-
 Candida albicans
3.6.1.42 GDPase
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.42 30
-
 assay at Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.42 7.5
-
 assay at Candida albicans