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Literature summary extracted from
- Purification, properties, and partial amino acid sequences of alanine racemase from the muscle of the black tiger prawn Penaeus monodon (2002), Comp. Biochem. Physiol. B, 133, 445-453.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.1 | pyridoxal 5'-phosphate | 0.02 mM, activation to 108% of control in the direction of D-Ala formation, activation to 124% of control in the direction of L-Ala formation | Penaeus monodon |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.1 | Aminooxyacetate | 1 mM, complete inhibition, both directions | Penaeus monodon | |
| 5.1.1.1 | hydroxylamine | 1 mM, complete inhibition, both directions | Penaeus monodon | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.1 | 167 | - |
L-Ala | pH 8.5, 37°C | Penaeus monodon | |
| 5.1.1.1 | 179 | - |
D-Ala | pH 8.5, 37°C | Penaeus monodon | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 44000 | - |
2 * 44000, SDS-PAGE | Penaeus monodon |
| 5.1.1.1 | 90000 | - |
gel filtration | Penaeus monodon |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.1 | Penaeus monodon | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.1 | - |
Penaeus monodon |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.1.1.1 | muscle | - |
Penaeus monodon | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 2807 | - |
- |
Penaeus monodon |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.1 | L-Ala | - |
Penaeus monodon | D-Ala | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.1 | dimer | 2 * 44000, SDS-PAGE | Penaeus monodon |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 37 | - |
formation of L-Ala or D-Ala | Penaeus monodon |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 25 | 50 | 25°C: about 65% of maximal activity, formation of L-Ala or D-Ala, 50°C: about 50% of maximal activity in formation of D-Ala, about 60% of maximal activity in formation of L-Ala | Penaeus monodon |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.1 | 2314 | - |
D-Ala | pH 8.5, 37°C | Penaeus monodon | |
| 5.1.1.1 | 2589 | - |
L-Ala | pH 8.5, 37°C | Penaeus monodon | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 9.5 | - |
formation of D-Ala | Penaeus monodon |
| 5.1.1.1 | 10 | - |
formation of L-Ala | Penaeus monodon |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 7 | 11 | pH 7.0: about 40% of maximal activity in formation of D-Ala, about 50% of maximal activity in formation of L-Ala, pH 11: about 95% of maximal activity in formation of L-Ala and D-Ala | Penaeus monodon |
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