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Literature summary extracted from

  • Yoon, S.Y.; Noh, H.S.; Kim, E.H.; Kong, K.H.
    The highly stable alcohol dehydrogenase of Thermomicrobium roseum: purification and molecular characterization (2002), Comp. Biochem. Physiol. B, 132, 415-422.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 acetaldehyde strong product inhibition Thermomicrobium roseum
1.1.1.1 imidazole weak inhibition Thermomicrobium roseum
1.1.1.1 pyrazole strong inhibition Thermomicrobium roseum
1.1.1.1 Pyridine
-
 Thermomicrobium roseum
1.1.1.1 trichloroethanol weak inhibition Thermomicrobium roseum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 24.2
-
 ethanol pH 10.0, 50°C Thermomicrobium roseum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 43000
-
 2 * 43000, SDS-PAGE Thermomicrobium roseum
1.1.1.1 88000
-
 approximately, gel filtration Thermomicrobium roseum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Thermomicrobium roseum
-
 ATCC 27502
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 128fold to homogeneity by adenosine 5'-monophosphate affinity and Mono-Q ion exchange chromatography Thermomicrobium roseum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 1.28
-
 purified enzyme Thermomicrobium roseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 2,2,2-trichloroethanol + NAD+
-
 Thermomicrobium roseum trichloroacetaldehyde + NADH + H+
-
 ?
1.1.1.1 crotyl alcohol + NAD+
-
 Thermomicrobium roseum crotonaldehyde + NADH
-
 ?
1.1.1.1 ethanol + NAD+ best substrate Thermomicrobium roseum acetaldehyde + NADH
-
 r
1.1.1.1 methanol + NAD+ low activity Thermomicrobium roseum formaldehyde + NADH + H+
-
 ?
1.1.1.1 additional information enzyme shows high substrate specificity towards primary aliphatic alcohols, no activity with 2-butanol, tert-butanol, isoamyl alcohol, isobutyl alcohol, 1,6-hexadiol, and mono-, di-, and triethanolamine Thermomicrobium roseum ?
-
 ?
1.1.1.1 n-butanol + NAD+ low activity Thermomicrobium roseum butanal + NADH
-
 ?
1.1.1.1 n-propanol + NAD+
-
 Thermomicrobium roseum propionaldehyde + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 43000, SDS-PAGE Thermomicrobium roseum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH
-
 Thermomicrobium roseum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 70
-
-
 Thermomicrobium roseum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 30 85 maximal activity at 70°C, 60% of maximal activity at 85°C Thermomicrobium roseum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 70
-
 stable upt to Thermomicrobium roseum
1.1.1.1 75
-
 and above, rapid loss of activity Thermomicrobium roseum
1.1.1.1 85
-
 slight residual activity Thermomicrobium roseum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 10
-
-
 Thermomicrobium roseum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Thermomicrobium roseum
1.1.1.1 NADH
-
 Thermomicrobium roseum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.1 Thermomicrobium roseum
-
-
 6