EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.268 | ethanesulfonate | 1 mM, 5.6fold increase in ratio of turnover number to Km-value for 2-butanone as substrate | Xanthobacter autotrophicus | |
1.1.1.268 | methanesulfonate | 1 mM, 5.6fold increase in ratio of turnover number to Km-value for 2-butanone as substrate | Xanthobacter autotrophicus | |
1.1.1.268 | propanesulfonate | 1 mM, 1.7fold increase in ratio of turnover number to Km-value for 2-butanone as substrate | Xanthobacter autotrophicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.268 | - |
Xanthobacter autotrophicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.268 | R152A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 5192fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.3fold higher than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
1.1.1.268 | R179A | no detectable activity with 2-(2-ketopropylthio)ethanesulfonate and 2-butanone | Xanthobacter autotrophicus |
1.1.1.268 | R196A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 1000fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 2.7fold higher than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
1.1.1.268 | R203A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 4.3fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.6fold lower than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
1.1.1.268 | R209A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 2.7fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.4fold lower than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.268 | 0.092 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
1.1.1.268 | 0.1 | - |
2-[(S)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 0.102 | - |
2-[(R)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 0.44 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
1.1.1.268 | 1.08 | - |
(R)-2-octanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 2 | 3 | 2-butanone | pH 7.5, 30°C, mutant enzyme R196A | Xanthobacter autotrophicus | |
1.1.1.268 | 2.64 | - |
(R)-2-heptanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 4.6 | - |
(R)-2-hexanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 20 | - |
(R)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 39 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R203A | Xanthobacter autotrophicus | |
1.1.1.268 | 41 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R152A | Xanthobacter autotrophicus | |
1.1.1.268 | 52 | - |
2-butanone | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
1.1.1.268 | 84 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
1.1.1.268 | 153 | - |
(S)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 315 | - |
(S)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 328 | - |
(R)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 1726 | - |
2-propanol | pH 7.5, 30°C | Xanthobacter autotrophicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.268 | Xanthobacter autotrophicus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.268 | (R)-2-butanol + NAD+ | the enantiomeric selectivity of the reverse reaction is 39.6% for (S)-2-butanol without additive, 90.8% with 1 mM CH3CH2SO3-Na+ as additive, 87.8% with 1 mM HSCH2CH2SO3-Na+ as additive, 52.0% with 1 mM CH3CH2COO-Na+ as additive, 46.6% with 1 mM CH3COO-Na+ as additive, 44.8% with 1 mM CH3CH2NH3+Cl- as additive, 45.4% with 1 mM CH3CH2OH as additive, 45.8% with 1 mM Na2SO4 as additive and 40.4% with 1 mM NaCl as additive | Xanthobacter autotrophicus | 2-butanone + NADH + H+ | - |
r | |
1.1.1.268 | (R)-2-heptanol + NAD+ | - |
Xanthobacter autotrophicus | 2-heptanone + NADH + H+ | - |
? | |
1.1.1.268 | (R)-2-hexanol + NAD+ | - |
Xanthobacter autotrophicus | 2-hexanone + NADH + H+ | - |
? | |
1.1.1.268 | (R)-2-octanol + NAD+ | - |
Xanthobacter autotrophicus | 2-octanone + NADH + H+ | - |
? | |
1.1.1.268 | (R)-2-pentanol + NAD+ | - |
Xanthobacter autotrophicus | 2-pentanone + NADH + H+ | - |
? | |
1.1.1.268 | (S)-2-butanol + NAD+ | - |
Xanthobacter autotrophicus | 2-butanone + NADH + H+ | - |
? | |
1.1.1.268 | (S)-2-pentanol + NAD+ | - |
Xanthobacter autotrophicus | 2-pentanone + NADH + H+ | - |
? | |
1.1.1.268 | 2-propanol + NAD+ | - |
Xanthobacter autotrophicus | acetone + NADH + H+ | - |
? | |
1.1.1.268 | 2-[(R)-2-hydroxypropylthio]ethanesulfonate + NAD+ | the enzyme is highly specific for the R-enantiomer | Xanthobacter autotrophicus | 2-(2-ketopropylthio)ethanesulfonate + NADH + H+ | - |
r | |
1.1.1.268 | 2-[(S)-2-hydroxypropylthio]ethanesulfonate + NAD+ | the enzyme is highly specific for the R-enantiomer | Xanthobacter autotrophicus | 2-(2-ketopropylthio)ethanesulfonate + NADH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.268 | R-HPCDH | - |
Xanthobacter autotrophicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.268 | 0.012 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R203A | Xanthobacter autotrophicus | |
1.1.1.268 | 0.027 | - |
2-butanone | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
1.1.1.268 | 0.028 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R152A | Xanthobacter autotrophicus | |
1.1.1.268 | 0.032 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
1.1.1.268 | 0.033 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R196A | Xanthobacter autotrophicus | |
1.1.1.268 | 0.044 | - |
2-[(S)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 0.98 | - |
(R)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 1.1 | - |
(R)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 1.4 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R152A | Xanthobacter autotrophicus | |
1.1.1.268 | 1.7 | - |
(R)-2-octanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 1.8 | - |
(R)-2-heptanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 2.2 | - |
(S)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 2.4 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R196A | Xanthobacter autotrophicus | |
1.1.1.268 | 2.7 | - |
(R)-2-hexanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 2.9 | - |
2-propanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 2.9 | - |
(S)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 15 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
1.1.1.268 | 24.5 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
1.1.1.268 | 26.8 | - |
2-[(R)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1.1.268 | 27.9 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R203A | Xanthobacter autotrophicus |