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Literature summary extracted from

  • Usta, J.; El Bawab, S.; Roddy, P.; Szulc, Z.M.; Yusuf; Hannun, A.; Bielawska, A.
    Structural requirements of ceramide and sphingosine based inhibitors of mitochondrial ceramidase (2001), Biochemistry, 40, 9657-9668.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 ceramide-1-phosphate stimulates Rattus norvegicus
3.5.1.23 sphingosine-1-phosphate stimulates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 (2S)-3-keto-dehydrosphingosine mtCDase, weak inhibition Rattus norvegicus
3.5.1.23 (2S)-3-keto-hexadecanoyl-ceramide mtCDase, IC50: 0.6 mol% Rattus norvegicus
3.5.1.23 (2S)-3-keto-sphinganine mtCDase, IC50: 0.34 mol% Rattus norvegicus
3.5.1.23 1-O-methyl-D-erythro-sphingosine mtCDase, weak inhibition Rattus norvegicus
3.5.1.23 C18-ceramide mtCDase, competitive inhibition, IC50: 0.62 mol% Rattus norvegicus
3.5.1.23 cis-D-erythro-sphingosine mtCDase, weak inhibition Rattus norvegicus
3.5.1.23 D-erythro-dehydrosphingosine mtCDase, IC50: 0.25 mol% Rattus norvegicus
3.5.1.23 D-erythro-sphinganine mtCDase, IC50: 0.2 mol% Rattus norvegicus
3.5.1.23 D-erythro-sphingosine IC50: 0.04 mol%, mt-CDase is inhibited by all stereoisomers of sphingosine with IC50 ranging from 0.04 to 0.14 mol% Rattus norvegicus
3.5.1.23 D-erythro-urea-C16-ceramide mtCDase, competitive inhibition, IC50: 0.33 mol% Rattus norvegicus
3.5.1.23 D-threo-ceramide mt-CDase, IC50: 0.21 mol% Rattus norvegicus
3.5.1.23 L-erythro-ceramide mt-CDase, IC50: 0.26 mol% Rattus norvegicus
3.5.1.23 L-threo-ceramide mt-CDase, IC50: 0.11 mol% Rattus norvegicus
3.5.1.23 additional information not inhibited by N-methyl-hexadecanoyl-ceramide, 1-O-methyl-hexadecanoyl-ceramide, 3-O-methyl-hexadecanoyl-ceramide, cis-D-erythro-hexadecanoyl-ceramide, 3-O-methyl-D-erythro-sphingosine, requirements for inhibition of mtCDase Rattus norvegicus
3.5.1.23 N,N-dimethyl-D-erythro-sphingosine mtCDase, weak inhibition Rattus norvegicus
3.5.1.23 N-methyl-D-erythro-sphingosine mtCDase, IC50: 0.13 mol% Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 additional information
-
 additional information
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 mitochondrion
-
 Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 D-erythro-octadecanoyl-ceramide + H2O mtCDase specifically hydrolyzes the D-erythro-isomer of ceramide, requirements for ceramide-enzyme interaction, ligand interaction with the enzyme occurs in a high affinity low specificity manner, in contrast to catalysis which is highly specific for D-erythro-ceramide but occurs with a lower affinity Rattus norvegicus D-erythro-sphingosine + palmitic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 mt-CDase mitochondrial ceramidase Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 9.5
-
 assay at Rattus norvegicus