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Literature summary extracted from

  • Kumar, K.; Walz, F.G., Jr.
    Probing functional perfection in substructures of ribonuclease T1: double combinatorial random mutagenesis involving Asn43, Asn44, and Glu46 in the guanine binding loop (2001), Biochemistry, 40, 3748-3757.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.24 expression in Escherichia coli Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.24 N43G as active as the wild type enzyme Aspergillus oryzae
4.6.1.24 N43H as active as the wild type enzyme Aspergillus oryzae
4.6.1.24 N43I as active as the wild type enzyme Aspergillus oryzae
4.6.1.24 N43Q/N44G less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 N43T as active as the wild type enzyme Aspergillus oryzae
4.6.1.24 N43Y as active as the wild type enzyme Aspergillus oryzae
4.6.1.24 N44H less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_G47insL mutant with a Leu insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_N47insQ mutant with a Glu insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_P47insP mutant with a Pro insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_P47insQ mutant with a Glu insertion in 47, as active as the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_P47insY mutant with a Tyr insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_Q47insP mutant with a Pro insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_R47insR mutant with an Arg insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_S47insG mutant with a Gly insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_S47insS mutant with a Ser insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Q46_T47insS mutant with a Ser insertion in 47, less active than the wild type enzyme Aspergillus oryzae
4.6.1.24 Y45S as active as the wild type enzyme Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.24 0.052
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43H mutant Aspergillus oryzae
4.6.1.24 0.0581
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43G mutant Aspergillus oryzae
4.6.1.24 0.0757
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Q46_P47insQ mutant Aspergillus oryzae
4.6.1.24 0.0758
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43I mutant Aspergillus oryzae
4.6.1.24 0.0772
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43T mutant Aspergillus oryzae
4.6.1.24 0.081
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43Q/N44G mutant Aspergillus oryzae
4.6.1.24 0.092
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43T mutant Aspergillus oryzae
4.6.1.24 0.135
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, wild-type enzyme Aspergillus oryzae
4.6.1.24 0.156
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N44H mutant Aspergillus oryzae
4.6.1.24 0.17
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, Y45S mutant Aspergillus oryzae
4.6.1.24 0.173
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43H mutant Aspergillus oryzae
4.6.1.24 0.19
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N44H mutant Aspergillus oryzae
4.6.1.24 0.259
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43Y mutant Aspergillus oryzae
4.6.1.24 0.321
-
 RNA pH 7.5, 25ÂșC, wild-type enzyme Aspergillus oryzae
4.6.1.24 0.361
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Q46_Q47insP mutant Aspergillus oryzae
4.6.1.24 0.433
-
 RNA pH 7.5, 25ÂșC, Y45S Aspergillus oryzae
4.6.1.24 0.44
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, wild-type enzyme Aspergillus oryzae
4.6.1.24 0.506
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Q46_P47insP mutant Aspergillus oryzae
4.6.1.24 0.549
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43Q/N44G mutant Aspergillus oryzae
4.6.1.24 0.606
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Y45S mutant Aspergillus oryzae
4.6.1.24 0.61
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43I mutant Aspergillus oryzae
4.6.1.24 0.698
-
 RNA pH 7.5, 25ÂșC, N43H Aspergillus oryzae
4.6.1.24 0.73
-
 RNA pH 7.5, 25ÂșC, N43G Aspergillus oryzae
4.6.1.24 0.961
-
 RNA pH 7.5, 25ÂșC, N43Y Aspergillus oryzae
4.6.1.24 1.55
-
 RNA pH 7.5, 25ÂșC, N43I Aspergillus oryzae
4.6.1.24 2.64
-
 RNA pH 7.5, 25ÂșC, N43T Aspergillus oryzae
4.6.1.24 4
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43G mutant Aspergillus oryzae
4.6.1.24 4.56
-
 RNA pH 7.5, 25ÂșC, N43Q/N44G Aspergillus oryzae
4.6.1.24 6.94
-
 RNA pH 7.5, 25ÂșC, Q46PQ mutant Aspergillus oryzae
4.6.1.24 19.6
-
 RNA pH 7.5, 25ÂșC, N44H Aspergillus oryzae
4.6.1.24 1470
-
 RNA pH 7.5, 25ÂșC, Q46PP mutant Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.24 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.24 ion-exchange chromatography and gel filtration Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.24 adenylyl-3',5'-cytidine ApC Aspergillus oryzae adenosine-2',3'-cyclic phosphate + cytidine
-
 ?
4.6.1.24 ApC + H2O
-
 Aspergillus oryzae adenosine 3'-phosphate + cytidine
-
 ?
4.6.1.24 GpC + H2O
-
 Aspergillus oryzae guanosine 3'-phosphate + cytidine
-
 ?
4.6.1.24 guanylyl-3',5'-cytidine + H2O GpC Aspergillus oryzae guanosine 3'-phosphate + cytidine
-
 ?
4.6.1.24 RNA + H2O
-
 Aspergillus oryzae Hydrolyzed RNA
-
 ?