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Literature summary extracted from

  • Sanchez-Torres, P.; Visser, J.; Benen, J.A.
    Identification of amino acid residues critical for catalysis and stability in Aspergillus niger family 1 pectin lyase A (2003), Biochem. J., 370, 331-337.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.10 expression of mutant enzymes in Aspergillus niger strain NW188, subcloning in Escherichia coli DH5alpha Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.10 D154A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Aspergillus niger
4.2.2.10 D154E site-directed mutagenesis, reduced activity compared to the wild-type enzyme Aspergillus niger
4.2.2.10 D186N site-directed mutagenesis, reduced activity and altered pH profile compared to the wild-type enzyme Aspergillus niger
4.2.2.10 D186N/D221N site-directed mutagenesis, reduced activity and altered pH profile compared to the wild-type enzyme Aspergillus niger
4.2.2.10 D221N site-directed mutagenesis, reduced activity and altered pH profile compared to the wild-type enzyme Aspergillus niger
4.2.2.10 K239N site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Aspergillus niger
4.2.2.10 N109A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Aspergillus niger
4.2.2.10 R176A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Aspergillus niger
4.2.2.10 R176D site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Aspergillus niger
4.2.2.10 R176K site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Aspergillus niger
4.2.2.10 R236A site-directed mutagenesis, active site residue exchange, completely inactive mutant Aspergillus niger
4.2.2.10 R236K site-directed mutagenesis, active site residue exchange, completely inactive mutant Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.10 additional information
-
 additional information kinetics of wild-type and mutant enzymes, influence of pH on the kinetics Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 45000
-
 x * 45000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Aspergillus niger Q01172 family 1 pectin lyase A
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.10 glycoprotein the wild-type enzyme is N-glycosylated Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10 native wild-type enzyme and recombinant mutant enzymes to homogeneity Aspergillus niger

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.10 [6-O-methyl-alpha-D-GalpA]n = [6-O-methyl-alpha-D-GalpA]m + 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1 reaction mechanism, catalytic residues Arg236, Arg176, and Lys239 are essentially involved in catalysis Aspergillus niger
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 additional information pH driven conformational changes of the enzyme, wild-type and mutants Aspergillus niger ?
-
 ?
4.2.2.10 partially methyl-esterified pectin prepared from citrus and Aspergillus sp. Aspergillus niger ?
-
 ?
4.2.2.10 pectin citrus pectin Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.10 ? x * 45000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 family 1 pectin lyase A
-
 Aspergillus niger
4.2.2.10 PL1A
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 30
-
 assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 5
-
 mutant D186N Aspergillus niger
4.2.2.10 6
-
 wild-type enzyme Aspergillus niger
4.2.2.10 8
-
 mutants D221N and D186N/D221N Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.10 5 8
-
 Aspergillus niger