Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hahn, G.; Kaup, B.; Bringer-Meyer, S.; Sahm, H.
    A zinc-containing mannitol-2-dehydrogenase from Leuconostoc pseudomesenteroides ATCC 12291: purification of the enzyme and cloning of the gene (2003), Arch. Microbiol., 179, 101-107.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.67 expression in Escherichia coli Leuconostoc pseudomesenteroides

General Stability

EC Number General Stability Organism
1.1.1.67 DTT stabilizes Leuconostoc pseudomesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.67 citrate 50 mM, pH 5.4, 72% inhibition Leuconostoc pseudomesenteroides
1.1.1.67 EDTA 20 mM, complete inhibition, can be restored by 20 mM Zn2+, but not by Mn2+ or Mg2+ Leuconostoc pseudomesenteroides
1.1.1.67 imidazole 175 mM,pH 7.0, 74% inhibition Leuconostoc pseudomesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.67 12
-
 D-mannitol pH 8.6, 30°C Leuconostoc pseudomesenteroides
1.1.1.67 44
-
 D-fructose pH 5.4, 30°C Leuconostoc pseudomesenteroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.67 Zn2+ cofactor Leuconostoc pseudomesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.67 43000
-
 4 * 43000, SDS-PAGE Leuconostoc pseudomesenteroides
1.1.1.67 155000
-
 gel filtration Leuconostoc pseudomesenteroides

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Leuconostoc pseudomesenteroides Q83VI5 ATCC 12291
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.67
-
 Leuconostoc pseudomesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.67 450
-
-
 Leuconostoc pseudomesenteroides

Storage Stability

EC Number Storage Stability Organism
1.1.1.67 4°C or 20°C, enzyme loses approximately 60% of its specific activity after storage for 3 weeks in presence of 1 mM DTT, without DTT the same loss is observed after 2 days Leuconostoc pseudomesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-fructose + NADH + H+
-
 Leuconostoc pseudomesenteroides D-mannitol + NAD+
-
 r
1.1.1.67 D-glucitol + NAD+ 4% of the activity with D-mannitol Leuconostoc pseudomesenteroides ?
-
 ?
1.1.1.67 D-mannitol + NADP+ 32% of the activity with NAD+ Leuconostoc pseudomesenteroides D-fructose + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.67 tetramer 4 * 43000, SDS-PAGE Leuconostoc pseudomesenteroides

Synonyms

EC Number Synonyms Comment Organism
1.1.1.67 mannitol 2-dehydrogenase
-
 Leuconostoc pseudomesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.67 5.4
-
 reduction of D-fructose Leuconostoc pseudomesenteroides
1.1.1.67 8.6
-
 oxidation of D-mannitol Leuconostoc pseudomesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.67 NAD+
-
 Leuconostoc pseudomesenteroides
1.1.1.67 NADH
-
 Leuconostoc pseudomesenteroides
1.1.1.67 NADP+ 32% of the activity with NAD+ Leuconostoc pseudomesenteroides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.67 Leuconostoc pseudomesenteroides chromatofocusing
-
 4.2