EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.77 | expression in Escherichia coli as a fusion protein with maltose-binding protein | Arabidopsis thaliana |
1.3.1.77 | expression in Escherichia coli as a fusion protein with maltose-binding protein | Medicago truncatula |
1.17.1.3 | expression in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
1.17.1.3 | expression in Escherichia coli strain DH5alpha, expression as MBT-fusion protein followed by cleavage of the protein tag by Factor Xa protease | Medicago truncatula |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.77 | (+)-catechin | 0.5 mM, 50% inhibition | Arabidopsis thaliana | |
1.3.1.77 | (+/-)-dihydroquercetin | 0.025 mM | Arabidopsis thaliana | |
1.3.1.77 | additional information | no inhibition by Na+ up to 400 mM | Arabidopsis thaliana | |
1.3.1.77 | additional information | no inhibition by (+)-catechin and (+/-)-dihydroquercetin | Medicago truncatula | |
1.3.1.77 | Na+ | above 200 mM | Medicago truncatula | |
1.3.1.77 | quercetin | strong inhibition | Arabidopsis thaliana | |
1.3.1.77 | quercetin | strong inhibition | Medicago truncatula | |
1.17.1.3 | (+)-catechin | - |
Arabidopsis thaliana | |
1.17.1.3 | cyanidin | substrate inhibition at high concentration | Arabidopsis thaliana | |
1.17.1.3 | dihydroquercetin | - |
Arabidopsis thaliana | |
1.17.1.3 | additional information | no inhibition by (+)-catechin and dihydroquercetin | Medicago truncatula | |
1.17.1.3 | Na+ | above 200 mM | Medicago truncatula | |
1.17.1.3 | pelargonidin | substrate inhibition at high concentration | Arabidopsis thaliana | |
1.17.1.3 | quercetin | - |
Arabidopsis thaliana | |
1.17.1.3 | quercetin | - |
Medicago truncatula |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.77 | 0.0129 | - |
cyanidin | pH 6.0 | Medicago truncatula | |
1.3.1.77 | 0.0145 | - |
pelargonidin | pH 6.0 | Medicago truncatula | |
1.3.1.77 | 0.0178 | - |
delphinidin | pH 8.0 | Arabidopsis thaliana | |
1.3.1.77 | 0.0498 | - |
delphinidin | pH 6.0 | Medicago truncatula | |
1.3.1.77 | 0.0528 | - |
pelargonidin | pH 8.0 | Arabidopsis thaliana | |
1.3.1.77 | 0.0739 | - |
cyanidin | pH 8.0 | Arabidopsis thaliana | |
1.3.1.77 | 0.45 | - |
NADPH | pH 6.0 | Medicago truncatula | |
1.3.1.77 | 0.94 | - |
NADH | pH 6.0 | Medicago truncatula | |
1.17.1.3 | additional information | - |
additional information | - |
Arabidopsis thaliana | |
1.17.1.3 | additional information | - |
additional information | enzyme shows typical Michaelis-Menten kinetics for each substrate | Medicago truncatula |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.3 | additional information | enzyme is not affected by Na+ at up to 400 mM | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.77 | anthocyanidin + NAD(P)H | Arabidopsis thaliana | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? | |
1.3.1.77 | anthocyanidin + NAD(P)H | Medicago truncatula | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? | |
1.17.1.3 | cyanidin + NADPH + H+ | Arabidopsis thaliana | - |
(-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
1.17.1.3 | cyanidin + NADPH + H+ | Medicago truncatula | - |
(-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
1.17.1.3 | additional information | Arabidopsis thaliana | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | ? | - |
? | |
1.17.1.3 | additional information | Medicago truncatula | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.77 | Arabidopsis thaliana | - |
- |
- |
1.3.1.77 | Medicago truncatula | - |
- |
- |
1.17.1.3 | Arabidopsis thaliana | - |
cv. Columbia, gene BANYULS | - |
1.17.1.3 | Medicago truncatula | - |
gene BANYULS | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.3 | recombinant enzyme from Escherichia coli | Arabidopsis thaliana |
1.17.1.3 | recombinant enzyme from Escherichia coli | Medicago truncatula |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.77 | (-)-epicatechin + NADH + H+ | - |
Medicago truncatula | cyanidin + NAD+ | - |
r | |
1.3.1.77 | anthocyanidin + NAD(P)H | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | Arabidopsis thaliana | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? | |
1.3.1.77 | anthocyanidin + NAD(P)H | enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins | Medicago truncatula | 2,3-cis-flavan-3-ol + NAD(P)+ | - |
? | |
1.3.1.77 | cyanidin + NADPH | - |
Arabidopsis thaliana | (-)-epicatechin + NAD(P)+ | - |
? | |
1.3.1.77 | cyanidin + NADPH | preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin | Medicago truncatula | (-)-epicatechin + NAD(P)+ | - |
? | |
1.3.1.77 | delphinidin + 2 NADPH + H+ | - |
Arabidopsis thaliana | (-)-epigallocatechin + 2 NADP+ | - |
? | |
1.3.1.77 | delphinidin + 2 NADPH + H+ | preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin | Medicago truncatula | (-)-epigallocatechin + 2 NADP+ | - |
? | |
1.3.1.77 | pelargonidin + 2 NADPH + H+ | - |
Arabidopsis thaliana | (-)-epiafzelechin + 2 NADP+ | - |
? | |
1.3.1.77 | pelargonidin + 2 NADPH + H+ | preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin | Medicago truncatula | (-)-epiafzelechin + 2 NADP+ | - |
? | |
1.17.1.3 | cyanidin + NADPH + H+ | - |
Arabidopsis thaliana | (-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
1.17.1.3 | cyanidin + NADPH + H+ | - |
Medicago truncatula | (-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
1.17.1.3 | cyanidin + NADPH + H+ | - |
Arabidopsis thaliana | (-)-epicatechin + (-)-catechin + NADP+ + H2O | (-)-epicatechin, i.e. (2R,3R)-3-cis-flavan-3-ol, is the major product, formation of (-)-catechin by nonenzymatic epimerization is possible | ? | |
1.17.1.3 | cyanidin + NADPH + H+ | - |
Medicago truncatula | (-)-epicatechin + (-)-catechin + NADP+ + H2O | (-)-epicatechin, i.e. (2R,3R)-3-cis-flavan-3-ol, is the major product, formation of (-)-catechin by nonenzymatic epimerization is possible | ? | |
1.17.1.3 | delphinidin + NADPH | - |
Arabidopsis thaliana | (-)-epigallocatechin + (-)-gallocatechin + NADP+ + H2O | (-)-epigallocatechin is the major product, formation of (-)-gallocatechin by nonenzymatic epimerization is possible | ? | |
1.17.1.3 | delphinidin + NADPH | - |
Medicago truncatula | (-)-epigallocatechin + (-)-gallocatechin + NADP+ + H2O | (-)-epigallocatechin is the major product, formation of (-)-gallocatechin by nonenzymatic epimerization is possible | ? | |
1.17.1.3 | additional information | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | Arabidopsis thaliana | ? | - |
? | |
1.17.1.3 | additional information | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | Medicago truncatula | ? | - |
? | |
1.17.1.3 | additional information | substrate preference in descending order: cyanidin, pelargonidin, delphinidin | Medicago truncatula | ? | - |
? | |
1.17.1.3 | additional information | substrate preference in descending order: delphinidin, pelargonidin, cyanidin | Arabidopsis thaliana | ? | - |
? | |
1.17.1.3 | pelargonidin + NADPH | - |
Arabidopsis thaliana | (-)-epiafzelechin + (-)-afzelechin + NADP+ + H2O | (-)-epiafzelechin is the major product, formation of (-)-afzelechin by nonenzymatic epimerization is possible | ? | |
1.17.1.3 | pelargonidin + NADPH | - |
Medicago truncatula | (-)-epiafzelechin + (-)-afzelechin + NADP+ + H2O | (-)-epiafzelechin is the major product, formation of (-)-afzelechin by nonenzymatic epimerization is possible | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.77 | ANR | - |
Arabidopsis thaliana |
1.3.1.77 | ANR | - |
Medicago truncatula |
1.3.1.77 | AtANR | - |
Arabidopsis thaliana |
1.3.1.77 | MtANR | - |
Medicago truncatula |
1.17.1.3 | ANR | - |
Arabidopsis thaliana |
1.17.1.3 | ANR | - |
Medicago truncatula |
1.17.1.3 | anthocyanidin reductase | - |
Arabidopsis thaliana |
1.17.1.3 | anthocyanidin reductase | - |
Medicago truncatula |
1.17.1.3 | BAN | - |
Arabidopsis thaliana |
1.17.1.3 | BAN | - |
Medicago truncatula |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.77 | 45 | - |
- |
Medicago truncatula |
1.3.1.77 | 55 | - |
- |
Arabidopsis thaliana |
1.17.1.3 | 45 | - |
- |
Medicago truncatula |
1.17.1.3 | 55 | - |
- |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.77 | 0.058 | - |
delphinidin | pH 8.0 | Arabidopsis thaliana | |
1.3.1.77 | 0.18 | - |
cyanidin | pH 6.0 | Medicago truncatula | |
1.3.1.77 | 1.045 | - |
cyanidin | pH 8.0 | Arabidopsis thaliana | |
1.3.1.77 | 1.34 | - |
delphinidin | pH 6.0 | Medicago truncatula |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.77 | 5.5 | - |
in 50 mM citrate/phosphate buffer | Medicago truncatula |
1.3.1.77 | 6 | - |
in 50 mM MES buffer | Medicago truncatula |
1.3.1.77 | 8 | - |
- |
Arabidopsis thaliana |
1.17.1.3 | 5.5 | 6 | depending on the buffer system used | Medicago truncatula |
1.17.1.3 | 7 | - |
assay at | Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.77 | 7 | 8.9 | pH 7.0: about 40% of maximal activity, pH 8.9: about 90% of maximal activity | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.77 | NADH | slight preference for NADPH over NADH | Arabidopsis thaliana | |
1.3.1.77 | NADH | slight preference for NADPH over NADH | Medicago truncatula | |
1.3.1.77 | NADPH | slight preference for NADPH over NADH | Arabidopsis thaliana | |
1.3.1.77 | NADPH | slight preference for NADPH over NADH | Medicago truncatula | |
1.17.1.3 | NADH | - |
Medicago truncatula | |
1.17.1.3 | NADPH | slight preference for | Medicago truncatula | |
1.17.1.3 | NADPH | specific for, positive cooperativity | Arabidopsis thaliana |