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Literature summary extracted from

  • Szoor, B.; Gross, S.; Alphey, L.
    Biochemical characterization of recombinant Drosophila type 1 serine/threonine protein phosphatase (PP1c) produced in Pichia pastoris (2001), Arch. Biochem. Biophys., 396, 213-218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expression with a His tag in Pichia pastoris Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 Drosophila melanogaster protein phosphatase inhibitor-2 IC50: 0.5-1 nM Drosophila melanogaster
3.1.3.16 okadaic acid IC50: 40 nM Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 37000
-
 x * 37000, SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16
-
 Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 12.08
-
 after MonoQ FPLC purification step Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 p-nitrophenyl phosphate + H2O very low activity Drosophila melanogaster p-nitrophenol + phosphate
-
 ?
3.1.3.16 phosphorylated phosphorylase alpha + H2O
-
 Drosophila melanogaster phosphorylase alpha + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.16 ? x * 37000, SDS-PAGE Drosophila melanogaster

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.16 0.0000005 0.000001 IC50: 0.5-1 nM Drosophila melanogaster Drosophila melanogaster protein phosphatase inhibitor-2
3.1.3.16 0.00004
-
 IC50: 40 nM Drosophila melanogaster okadaic acid