Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Seeger, M.; Gonzalez, M.; Camara, B.; Munoz, L.; Ponce, E.; Mejias, L.; Mascayano, C.; Vasquez, Y.; Sepulveda-Boza, S.
    Biotransformation of natural and synthetic isoflavonoids by two recombinant microbial enzymes (2003), Appl. Environ. Microbiol., 69, 5045-5050.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.56 functional coexpression of enzymes BphB and BphA in Escherichia coli, gene construct bphA1A2A3A4B in the plasmid vector Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.56 7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. LB400 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 7-hydroxyisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 7-hydroxyisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. LB400 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 additional information Burkholderia sp. biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview ?
-
 ?
1.3.1.56 additional information Burkholderia sp. LB400 biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.56 Burkholderia sp.
-
 enzyme BphB
-
1.3.1.56 Burkholderia sp. LB400
-
 enzyme BphB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.56 7,2',3'-trihydroxy-8-methyl-2',3'-dihydro-isoflavone + NAD+ recombinant enzyme Burkholderia sp. 7,2',3'-trihydroxy-8-methylisoflavone + NADH
-
 ?
1.3.1.56 7,2',3'-trihydroxy-8-methyl-2',3'-dihydro-isoflavone + NAD+ recombinant enzyme Burkholderia sp. LB400 7,2',3'-trihydroxy-8-methylisoflavone + NADH
-
 ?
1.3.1.56 7,3',4'-trihydroxy-3',4'-dihydro-isoflavone + NAD+ recombinant enzyme Burkholderia sp. 7,3',4'-trihydroxyisoflavone + NADH
-
 ?
1.3.1.56 7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. LB400 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 7-hydroxyisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 7-hydroxyisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. LB400 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
1.3.1.56 additional information biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview Burkholderia sp. ?
-
 ?
1.3.1.56 additional information enzyme shows a broad substrate specificity Burkholderia sp. ?
-
 ?
1.3.1.56 additional information biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview Burkholderia sp. LB400 ?
-
 ?
1.3.1.56 additional information enzyme shows a broad substrate specificity Burkholderia sp. LB400 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.56 biphenyl-2,3-dihydrodiol 2,3-dehydrogenase
-
 Burkholderia sp.
1.3.1.56 BphB
-
 Burkholderia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.56 30
-
 assay at, cell culture temperature for in vivo experiments Burkholderia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.56 7.5
-
 assay at, medium for in vivo experiments Burkholderia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.56 NAD+
-
 Burkholderia sp.