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Literature summary extracted from

  • Lee, J.K.; Koo, B.S.; Kim, S.Y.; Hyun, H.H.
    Purification and characterization of a novel mannitol dehydrogenase from a newly isolated strain of Candida magnoliae (2003), Appl. Environ. Microbiol., 69, 4438-4447.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.138 2-mercaptoethanol 0.5 mM, complete inhibition Starmerella magnoliae
1.1.1.138 Cu2+
-
 Starmerella magnoliae
1.1.1.138 D-mannitol
-
 Starmerella magnoliae
1.1.1.138 dithiothreitol 0.5 mM, complete inhibition Starmerella magnoliae
1.1.1.138 glutathione 0.5 mM, complete inhibition Starmerella magnoliae
1.1.1.138 L-cysteine 0.5 mM, complete inhibition Starmerella magnoliae
1.1.1.138 NADP+
-
 Starmerella magnoliae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 0.0578
-
 NADPH pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 0.612
-
 NADH pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 28
-
 D-fructose pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 212
-
 ribose pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 352
-
 D-mannose pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 385
-
 arabinose pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 402
-
 xylose pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 680
-
 D-mannitol pH 7.5, 37°C Starmerella magnoliae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.138 additional information no stimulation by: MgCl2, MnCl2, ZnCl2, NiCl2, CoCl2, FeCl2 Starmerella magnoliae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 35000
-
 4 * 35000, SDS-PAGE Starmerella magnoliae
1.1.1.138 142000
-
 gel filtration Starmerella magnoliae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Starmerella magnoliae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138
-
 Starmerella magnoliae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.138 D-mannitol + NADP+ = D-fructose + NADPH + H+ sequential ordered bi-bi mechanism Starmerella magnoliae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 3.1
-
 substrate D-fructose + NADH, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 3.2
-
 substrate arabinose, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 5.1
-
 substrate xylose, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 5.2
-
 substrate ribose, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 6.1
-
 substrate D-mannose, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 9.1
-
 substrate D-mannitol, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 315
-
 substrate D-fructose + NADPH, pH 7.5, 37°C Starmerella magnoliae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 arabinose + NADPH + H+
-
 Starmerella magnoliae arabinitol + NADP+
-
 r
1.1.1.138 D-fructose + NADPH
-
 Starmerella magnoliae D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NAD+
-
 Starmerella magnoliae D-fructose + NADH + H+
-
 r
1.1.1.138 D-mannitol + NADP+ less than 3% the rate of fructose reduction Starmerella magnoliae D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannose + NADPH + H+
-
 Starmerella magnoliae D-mannitol + NADP+
-
 r
1.1.1.138 additional information specific for transfer of 4-pro-S hydrogen of NADPH Starmerella magnoliae ?
-
 ?
1.1.1.138 ribose + NADPH + H+
-
 Starmerella magnoliae ribitol + NADP+
-
 r
1.1.1.138 xylose + NADPH + H+
-
 Starmerella magnoliae xylitol + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.138 tetramer 4 * 35000, SDS-PAGE Starmerella magnoliae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.138 37
-
 reduction of fructose Starmerella magnoliae
1.1.1.138 40
-
 oxidation ofr fructose Starmerella magnoliae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 7.5
-
 reduction of fructose Starmerella magnoliae
1.1.1.138 10
-
 oxidation of fructose Starmerella magnoliae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.138 0.0011
-
 Cu2+ pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 0.18
-
 NADP+ cosubstrate fructose, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 0.21
-
 NADP+ cosubstrate NADPH, pH 7.5, 37°C Starmerella magnoliae
1.1.1.138 188
-
 mannitol pH 7.5, 37°C Starmerella magnoliae