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Literature summary extracted from
- Comparison of catalytic properties of free and immobilized cellobiase novozym 188 (2001), Appl. Biochem. Biotechnol., 91-93, 615-626.
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | cellobiose + H2O | Aspergillus niger | - |
2 beta-D-glucose | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Aspergillus niger | - |
novozym 188 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 9.5 | - |
50°C, pH 4.8 | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | cellobiose + H2O | - |
Aspergillus niger | 2 beta-D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | cellobiase | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | 75 | approx. 20% of maximal activity at 40°C and pH 4.5, approx. 15% of maximal activity at 70°C and pH 4.5 | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | 55 | no loss of activity after 240 min | Aspergillus niger |
| 3.2.1.21 | 60 | - |
approx. 15% loss of activity after 240 min | Aspergillus niger |
| 3.2.1.21 | 65 | - |
approx. 70% loss of activity after 240 min | Aspergillus niger |
| 3.2.1.21 | 70 | - |
complete loss of activity after 90 min | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4.5 | - |
- |
Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3 | 7 | approx. 30% of maximal activity at pH 3.0 and 65°C, approx. 5% of maximal activity at pH 6.0 and 65°C | Aspergillus niger |
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Release 2023.1 (January 2023)
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