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Literature summary extracted from

  • Taylor, L.P.; Strenge, D.; Miller, K.D.
    The role of glycosylation in flavonol-induced pollen germination (1998), Adv. Exp. Med. Biol., 439, 35-44.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.234 0.011
-
 kaempferol forward reaction Petunia sp.
2.4.1.234 0.013
-
 UDP cosubstrate kaempferol 3-O-beta-D-galactoside, reverse reaction Petunia sp.
2.4.1.234 0.039
-
 kaempferol 3-O-beta-D-galactoside reverse reaction Petunia sp.
2.4.1.234 0.318
-
 UDP-galactose cosubstrate kaempferol, forward reaction Petunia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.234 UDP-D-galactose + quercetin Petunia sp. role in pollen germination UDP + quercetin 3-O-beta-D-galactoside quercetin 3-O-beta-D-galactoside induces pollen germination via the reverse activity r
2.4.1.234 UDP-galactose + flavonol Petunia sp. F3GalTase controls the formation of glycosylated flavonols in pollen, enzyme also catalzes the reverse reaction, i.e the production of flavonol aglycone from the galactoside and UDP, and has therefore the potential to control the level of the bioactive flavonol species and as a result, pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes, control mechanisms of the reversible reaction UDP + flavonol 3-O-beta-D-galactoside
-
 r
2.4.1.234 UDP-galactose + kaempferol Petunia sp. role in pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes UDP + kaempferol 3-O-beta-D-galactoside kaempferol 3-O-beta-D-galactoside induces pollen germination via the reverse activity r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.234 Petunia sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.234
-
 Petunia sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.234 pollen surface localized Petunia sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.234 additional information not: UDP-glucose Petunia sp. ?
-
 ?
2.4.1.234 UDP-D-galactose + quercetin
-
 Petunia sp. UDP + quercetin 3-O-beta-D-galactoside
-
 r
2.4.1.234 UDP-D-galactose + quercetin role in pollen germination Petunia sp. UDP + quercetin 3-O-beta-D-galactoside quercetin 3-O-beta-D-galactoside induces pollen germination via the reverse activity r
2.4.1.234 UDP-galactose + flavonol F3GalTase controls the formation of glycosylated flavonols in pollen, enzyme also catalzes the reverse reaction, i.e the production of flavonol aglycone from the galactoside and UDP, and has therefore the potential to control the level of the bioactive flavonol species and as a result, pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes, control mechanisms of the reversible reaction Petunia sp. UDP + flavonol 3-O-beta-D-galactoside
-
 r
2.4.1.234 UDP-galactose + flavonol absolute requirement for UDP-galactose Petunia sp. UDP + flavonol 3-O-beta-D-galactoside
-
 r
2.4.1.234 UDP-galactose + kaempferol
-
 Petunia sp. UDP + kaempferol 3-O-beta-D-galactoside
-
 r
2.4.1.234 UDP-galactose + kaempferol role in pollen germination, the direction of the reaction could be controlled in vivo by localized hydronium ion changes Petunia sp. UDP + kaempferol 3-O-beta-D-galactoside kaempferol 3-O-beta-D-galactoside induces pollen germination via the reverse activity r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.234 5.5
-
 reverse reaction Petunia sp.
2.4.1.234 7.5
-
 forward reaction Petunia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.234 5.5 7.5 the activity of the forward reaction is 1.6fold higher at pH 7.5 than at 5.5, the activity of the reverse reaction is 8fold higher at pH 5.5 than at 7.5 Petunia sp.