Literature summary extracted from

Kopecny, D.; Briozzo, P.; Joly, N.; Houba-Herin, N.; Madzak, C.; Pethe, C.; Laloue, M.
Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays (2004), Acta Crystallogr. Sect. D, 60, 1500-1501.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.5.99.12	overexpression in yeast Yarrowia lipolytica	Zea mays

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.5.99.12	purified recombinant enzyme, 20 mg/ml protein with 0.5% w/v n-octyl-beta-D-glucopyranoside, hanging drop vapour diffusion method, 0.002 ml protein solution mixed with 0.002 ml reservoir solution containing 30% w/v PEG 1500, 0.1 M Tris-HCl, pH 7.0, 1 week, 20°C, X-ray diffraction structure determination and analysis at 1.95 A resolution	Zea mays

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.5.99.12	63000	-	x * 69000, recombinant enzyme, SDS-PAGE, x * 63000, native enzyme, SDS-PAGE	Zea mays
1.5.99.12	69000	-	x * 69000, recombinant enzyme, SDS-PAGE, x * 63000, native enzyme, SDS-PAGE	Zea mays

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.5.99.12	N6-(DELTA2-isopentenyl)adenine + FAD + H2O	Zea mays	oxidative degradation of cytokinins	adenine + 3-methylbut-2-enal + FADH2	-	ir

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.99.12	Zea mays	-	enzyme ZmCKO1	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.5.99.12	recombinant from yeast Yarrowia lipolytica by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography	Zea mays

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.5.99.12	30.6	-	purified recombinant enzyme	Zea mays

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.99.12	N6-(DELTA2-isopentenyl)adenine + FAD + H2O	-	Zea mays	adenine + 3-methylbut-2-enal + FADH2	-	ir
1.5.99.12	N6-(DELTA2-isopentenyl)adenine + FAD + H2O	oxidative degradation of cytokinins	Zea mays	adenine + 3-methylbut-2-enal + FADH2	-	ir

Subunits

EC Number	Subunits	Comment	Organism
1.5.99.12	?	x * 69000, recombinant enzyme, SDS-PAGE, x * 63000, native enzyme, SDS-PAGE	Zea mays

Synonyms

EC Number	Synonyms	Comment	Organism
1.5.99.12	CKO	-	Zea mays
1.5.99.12	cytokinin oxidase/dehydrogenase	-	Zea mays

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.99.12	FAD	covalently bound	Zea mays

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Release 2023.1 (January 2023)