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Literature summary extracted from

  • Markovic-Housley, Z.; Miglierini, G.; Soldatova, L.; Rizkallah, P.J.; Muller, U.; Schirmer, T.
    Crystal structure of hyaluronidase, a major allergen of bee venom (2000), Structure, 8, 1025-1035.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.35 medicine enzyme is the target for immunotherapy of allergen reaction Apis mellifera

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.35 DNA and amino acid sequence analysis, expression as active C-terminally His-tagged enzyme in insect cells via baculovirus infection Apis mellifera

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.35 8-10 mg/ml purified recombinant enzyme, with or wihtout hexameric hyaluronic acid, in 5 mM sodium acetate, pH 5.4, hanging drop vapour diffusion method, 20°C, for triclinic crystals, equal volume of protein solution with reservoir solution containing 30% PEG 8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M ammonium sulfate, equilibration against the reservoir solution, 6-8 months, for monoclinic crystal growth within several days, 10% PEG 6000, 1.0 M NaCl, 20 mM acetic acid, pH 4.2 is used for mixing and equilibration, X-ray diffraction structure determination and analysis at 1.6-2.7 A resolution Apis mellifera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.35 extracellular
-
 Apis mellifera
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.35 hyaluronate + H2O Apis mellifera
-
 N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 additional information Apis mellifera the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Apis mellifera Q08169 bee
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 recombinant His-tagged enzyme Apis mellifera

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.35 hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide enzyme is a beta-1,4-glucosidase, substrate binding groove, enzyme-substrate complex structure, catalytic groups and reaction mechanism Apis mellifera
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 venom
-
 Apis mellifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronate + H2O
-
 Apis mellifera N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 additional information the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans Apis mellifera ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 Hya
-
 Apis mellifera
3.2.1.35 hyaluronidase
-
 Apis mellifera