EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | amino acid determinatioin of the purified enzyme, high-level expression in Pichia pastoris GS115, subcloning in Escherichia coli DH5alpha | Geobacillus thermocatenulatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | 2-propanol | - |
Geobacillus thermocatenulatus | |
3.1.1.3 | acetone | - |
Geobacillus thermocatenulatus | |
3.1.1.3 | cholate | 1% w/v, 20% decrease in activity | Geobacillus thermocatenulatus | |
3.1.1.3 | methanol | - |
Geobacillus thermocatenulatus | |
3.1.1.3 | SDS | 1% w/v, 95% decrease in activity | Geobacillus thermocatenulatus | |
3.1.1.3 | Triton X-100 | 1% w/v, 30% decrease in activity | Geobacillus thermocatenulatus | |
3.1.1.3 | Tween 20 | 1% w/v, 7% decrease in activity | Geobacillus thermocatenulatus | |
3.1.1.3 | Tween 80 | 1% w/v, 6% decrease in activity | Geobacillus thermocatenulatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 43000 | - |
x * 43000, recombinant enzyme, SDS-PAGE | Geobacillus thermocatenulatus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.3 | 2-propanol | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 47% reduced activity | Geobacillus thermocatenulatus |
3.1.1.3 | Acetone | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity | Geobacillus thermocatenulatus |
3.1.1.3 | Methanol | 30% v/v, 1 h, 30°C, 0.1 M Tris buffer, pH 8.0, 16% reduced activity | Geobacillus thermocatenulatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Geobacillus thermocatenulatus | - |
- |
- |
3.1.1.3 | Geobacillus thermocatenulatus BTL2 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | large scale from Pichia pastoris culture supernatant, 660fold | Geobacillus thermocatenulatus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 23000 | - |
purified enzyme, substrate tributyrin | Geobacillus thermocatenulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Geobacillus thermocatenulatus | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Geobacillus thermocatenulatus BTL2 | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | triacetin + H2O | poor substrate | Geobacillus thermocatenulatus | diacetin + acetate | - |
? | |
3.1.1.3 | tributyrin + H2O | best substrate | Geobacillus thermocatenulatus | dibutyrin + butyrate | - |
? | |
3.1.1.3 | tributyrin + H2O | best substrate | Geobacillus thermocatenulatus BTL2 | dibutyrin + butyrate | - |
? | |
3.1.1.3 | tricaprin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dicaprin + decaprate | - |
? | |
3.1.1.3 | tricaproin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dicaproin + caproate | - |
? | |
3.1.1.3 | trilaurin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | dilaurin + laurate | - |
? | |
3.1.1.3 | trilaurin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus BTL2 | dilaurin + laurate | - |
? | |
3.1.1.3 | trimyristin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | dimyristin + myristate | - |
? | |
3.1.1.3 | triolein + H2O | - |
Geobacillus thermocatenulatus | diolein + oleate | - |
? | |
3.1.1.3 | triolein + H2O | - |
Geobacillus thermocatenulatus BTL2 | diolein + oleate | - |
? | |
3.1.1.3 | tripalmitin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus | dipalmitin + palmitate | - |
? | |
3.1.1.3 | tripalmitin + H2O | about 20% of the activity with tributyrin | Geobacillus thermocatenulatus BTL2 | dipalmitin + palmitate | - |
? | |
3.1.1.3 | tristearin + H2O | about 10% of the activity with tributyrin | Geobacillus thermocatenulatus | distearin + stearate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 43000, recombinant enzyme, SDS-PAGE | Geobacillus thermocatenulatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | lipase | - |
Geobacillus thermocatenulatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 65 | - |
with substrate tributyrin, pH 8.0 | Geobacillus thermocatenulatus |
3.1.1.3 | 75 | - |
with substrate triolein, pH 8.0 | Geobacillus thermocatenulatus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 35 | 80 | - |
Geobacillus thermocatenulatus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 50 | - |
stable up to, pH 8.0, 0.1 M Tris buffer | Geobacillus thermocatenulatus |
3.1.1.3 | 70 | - |
18% remaining activity | Geobacillus thermocatenulatus |
3.1.1.3 | 80 | - |
5% remaining activity | Geobacillus thermocatenulatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 7.5 | - |
with substrate tributyrin, 60°C | Geobacillus thermocatenulatus |
3.1.1.3 | 9 | - |
with substrate triolein, 60°C | Geobacillus thermocatenulatus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 5 | 9 | - |
Geobacillus thermocatenulatus |