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Literature summary extracted from

  • Karasawa, K.; Harada, A.; Satoh, N.; Inoue, K.; Setaka, M.
    Plasma platelet activating factor-acetylhydrolase (PAF-AH) (2003), Prog. Lipid Res., 42, 93-114.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.47 medicine plasma isozyme is a target of many clinical studies in inflammatory diseases, such as asthma, sepsis, and vascular diseases Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.47 plasma isozyme, DNA and amino acid sequence determination and analysis, gene maps at chromosome 6q12-21.1 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.47 additional information 4% naturally occurring deficiency of plasma isozyme in Japanese population can be complemented by the other isozymes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.47 diisopropylfluorophosphate plasma isozyme containing the GXSXG motif Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.47 0.012 0.0137 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.47 extracellular plasma isozyme Cavia porcellus
-
-
3.1.1.47 extracellular plasma isozyme Homo sapiens
-
-
3.1.1.47 intracellular isozymes Ib and II Homo sapiens 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.47 40000
-
 1 * 45000, plasma isozyme, 1 * 40000, isozyme II Homo sapiens
3.1.1.47 43000 67000 plasma isozyme, gel filtration Homo sapiens
3.1.1.47 45000
-
 1 * 45000, plasma isozyme, 1 * 40000, isozyme II Homo sapiens
3.1.1.47 58000 63000 plasma isozyme, gel filtration Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O Cavia porcellus
-
 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O Homo sapiens
-
 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 additional information Homo sapiens HDL-associated plasma isozyme may be involved in the protective role of HDL in artherosclerosis, plasma enzyme is associated to LDL, preferably LDL-5 and VLDL-1 particles, the enzyme contributes to the oxidation of LDL in increasing the content of lysoPAF, inflammatory and vascular diseases alter the plasma isozyme activity, enzyme is involved in several diseases and deficiencies, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Cavia porcellus
-
-
-
3.1.1.47 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.47 glycoprotein plasma isozyme, extensive N-glycosylation at Asn423 and Asn433 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.47 from plasma Cavia porcellus
3.1.1.47 plasma isozyme to homogeneity, solubilization from LDL Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.47 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate mechanism, substrates can only bind in the aqueous phase to the active site of the plasma isozyme at the membrane-water interphase, catalytic triad is formed by Ser273, His351, and Asp296 Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 blood increased level in subjects with chronic cholestasis Homo sapiens
-
3.1.1.47 brain isozyme Ib Homo sapiens
-
3.1.1.47 hematopoietic cell secretion of plasma isozyme Homo sapiens
-
3.1.1.47 hepatocyte secretion of plasma isozyme Homo sapiens
-
3.1.1.47 kidney isozyme II Homo sapiens
-
3.1.1.47 Kupffer cell
-
 Homo sapiens
-
3.1.1.47 liver isozyme II, reduced enzyme amount after liver transplantation Homo sapiens
-
3.1.1.47 macrophage secretion of plasma isozyme after cleavage of the signal peptide at Ala17/Val18, high enzyme activity during differentiation of monocytes to macrophages Homo sapiens
-
3.1.1.47 mast cell secretion of plasma isozyme Homo sapiens
-
3.1.1.47 plasma plasma isozyme Cavia porcellus
-
3.1.1.47 plasma plasma isozyme Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 1-palmitoyl-2(5-oxovaleroyl)-sn-glycero-3-phosphocholine + H2O plasma isozyme Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + 5-oxopentanoate
-
 ?
3.1.1.47 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O plasma isozyme Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonate
-
 ?
3.1.1.47 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O plasma isozyme Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + glutarate
-
 ?
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
-
 Cavia porcellus 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor PAF, hydrolysis of the acetyl ester at the sn-2 position, conversion to the inactive lysoPAF Cavia porcellus 1-alkyl-sn-glycero-3-phosphocholine + acetate i.e. lysoPAF ?
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor PAF, hydrolysis of the acetyl ester at the sn-2 position, conversion to the inactive lysoPAF Homo sapiens 1-alkyl-sn-glycero-3-phosphocholine + acetate i.e. lysoPAF ?
3.1.1.47 additional information plasma isozyme and isozyme II shows also transacetylase activity, broad substrate specificity, plasma isozyme and isozyme II prefer PAF analogues with sn-2 propionyl and butyroyl moieties, while isozyme Ib hardly hydrolyzes these phospholipids, plasma isozyme hydrolyzes preferentially phosphatidylcholine with C9 acyl chain, activity is increased in the presence of an aldehyde group at the omega-end of the acyl chain Homo sapiens ?
-
 ?
3.1.1.47 additional information HDL-associated plasma isozyme may be involved in the protective role of HDL in artherosclerosis, plasma enzyme is associated to LDL, preferably LDL-5 and VLDL-1 particles, the enzyme contributes to the oxidation of LDL in increasing the content of lysoPAF, inflammatory and vascular diseases alter the plasma isozyme activity, enzyme is involved in several diseases and deficiencies, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.47 monomer 1 * 45000, plasma isozyme, 1 * 40000, isozyme II Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.47 PAF-acetylhydrolase
-
 Cavia porcellus
3.1.1.47 PAF-acetylhydrolase
-
 Homo sapiens
3.1.1.47 PAF-AH
-
 Cavia porcellus
3.1.1.47 PAF-AH
-
 Homo sapiens
3.1.1.47 platelet-activating factor-acetylhydrolase
-
 Cavia porcellus
3.1.1.47 platelet-activating factor-acetylhydrolase
-
 Homo sapiens