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Literature summary extracted from
- Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase (2000), Proc. Natl. Acad. Sci. USA, 97, 5895-5900.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | dioleoyl-phosphatidylserine | 0.05 mM, 4fold activation | Homo sapiens |  |
| 3.1.4.12 | phosphatidylethanol | 0.05 mM, 2fold activation | Homo sapiens | |
| 3.1.4.12 | phosphatidylserine | 0.05 mM, 4fold activation | Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | cloning of cDNA | Homo sapiens |
| 3.1.4.12 | cloning of cDNA, expression in HEK293 kidney cells | Mus musculus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.4.12 | reducing agents such as dithiothreitol stablize nSMase2 during preparation | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | EDTA | 5 mM, complete inhibition, activity is comletely restored by the subsequent addition of 10 mM Mg2+ or Mn2+ | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.12 | membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Mg2+ | required for activity | Homo sapiens | |
| 3.1.4.12 | Mn2+ | can replace Mn2+ | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 71000 | - |
x * 71000, deduced from nucleotide sequence | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Homo sapiens | Q9NY59 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | brain | - |
Homo sapiens | - |
| 3.1.4.12 | liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | ? | x * 71000, deduced from nucleotide sequence | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | neutral sphingomyelinase 2 | - |
Homo sapiens |
| 3.1.4.12 | neutral sphingomylinase 2 | - |
Homo sapiens |
| 3.1.4.12 | nSMase2 | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 7.5 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 4.5 | 9 | approx. 5% of maximal activity at pH 4.5, approx. 15% of maximal activity at pH 9.0 | Homo sapiens |
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