EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.4.99.25 | vapor-phase diffusion method, crystal structure of TruB apoenzyme in the absence of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound forms of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity | Escherichia coli |
5.4.99.25 | vapor-phase diffusion method, crystal structure of TruB in complex with 17-base stem-loop of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound form of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.70 | Escherichia coli | - |
TruB | - |
4.2.1.70 | Escherichia coli TruB | - |
TruB | - |
4.2.1.70 | Thermotoga maritima | - |
TruB | - |
4.2.1.70 | Thermotoga maritima TruB | - |
TruB | - |
5.4.99.25 | Escherichia coli | P60340 | - |
- |
5.4.99.25 | Thermotoga maritima | Q9WZW0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.25 | - |
Escherichia coli |
5.4.99.25 | - |
Thermotoga maritima |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.4.99.25 | tRNA uridine55 = tRNA pseudouridine55 | this enzyme undergoes significant conformational changes on binding to its substrate. These conformational changes include the ordering of the thumb loop, which binds right into the RNA hairpin loop, and a 10° hinge movement of the C-terminal domain. TruB recognizes its RNA substrate through a combination of rigid docking and induced fit, with TruB first rigidly binding to its target and then maximizing the interaction by induced fit | Escherichia coli | |
5.4.99.25 | tRNA uridine55 = tRNA pseudouridine55 | this enzyme undergoes significant conformational changes on binding to its substrate. These conformational changes include the ordering of the thumb loop, which binds right into the RNA hairpin loop, and a 10° hinge movement of the C-terminal domain. TruB recognizes its RNA substrate through a combination of rigid docking and induced fit, with TruB first rigidly binding to its target and then maximizing the interaction by induced fit | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.25 | RNA pseudouridine synthase TruB | - |
Escherichia coli |
5.4.99.25 | RNA pseudouridine synthase TruB | - |
Thermotoga maritima |
5.4.99.25 | TruB | - |
Escherichia coli |
5.4.99.25 | TruB | - |
Thermotoga maritima |