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Literature summary extracted from

  • Hegeman, C.E.; Grabau, E.A.
    A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings (2001), Plant Physiol., 126, 1598-1608.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8
-
 Glycine max
3.1.3.26
-
 Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 ATP substrate inhibition above 5 mM Glycine max
3.1.3.8 additional information no substrate inhibition by 4-nitrophenyl phosphate up to 10 mM Glycine max
3.1.3.8 phytate substrate inhibition above 5 mM Glycine max
3.1.3.8 Polyphosphate substrate inhibition above 5 mM Glycine max
3.1.3.26 ATP substrate inhibition above 5 mM Glycine max
3.1.3.26 additional information no substrate inhibition by 4-nitrophenyl phosphate up to 10 mM Glycine max
3.1.3.26 phytate substrate inhibition above 5 mM Glycine max
3.1.3.26 Polyphosphate substrate inhibition above 5 mM Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.061
-
 myo-inositol hexakisphosphate
-
 Glycine max
3.1.3.8 1.7
-
 ATP
-
 Glycine max
3.1.3.8 3.3
-
 Polyphosphate
-
 Glycine max
3.1.3.8 4.3
-
 4-nitrophenyl phosphate
-
 Glycine max
3.1.3.26 0.061
-
 myo-inositol hexakisphosphate
-
 Glycine max
3.1.3.26 1.7
-
 ATP
-
 Glycine max
3.1.3.26 3.3
-
 Polyphosphate
-
 Glycine max
3.1.3.26 4.3
-
 4-nitrophenyl phosphate
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 130000
-
 gel filtration Glycine max
3.1.3.26 130000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Glycine max
-
 L. Merr.. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Glycine max
-
 L. Merr.. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein
-
 Glycine max
3.1.3.26 glycoprotein
-
 Glycine max

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Glycine max
3.1.3.26
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 cotyledon phytase synthesized early in germination persists for several days in the cotyledon Glycine max
-
3.1.3.26 cotyledon phytase synthesized early in germination persists for several days in the cotyledon Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 additional information
-
 32000 OD355/mg Glycine max
3.1.3.26 additional information
-
 32000 OD355/mg Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 4-nitrophenyl phosphate + H2O
-
 Glycine max 4-nitrophenol + phosphate
-
 ?
3.1.3.8 ATP + H2O
-
 Glycine max ADP + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Glycine max ? + phosphate
-
 ?
3.1.3.8 polyphosphate + H2O
-
 Glycine max ?
-
 ?
3.1.3.26 ATP + H2O
-
 Glycine max ?
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Glycine max ? + phosphate
-
 ?
3.1.3.26 p-nitrophenyl phosphate + H2O
-
 Glycine max p-nitrophenol + phosphate
-
 ?
3.1.3.26 polyphosphate + H2O
-
 Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 dimer 2 * 70000-72000, SDS-PAGE Glycine max
3.1.3.26 dimer 2 * 70000-72000, SDS-PAGE Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 58
-
-
 Glycine max
3.1.3.26 58
-
-
 Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 34 62 34°C: about 55% of maximal activity, 62°C: about 75% of maximal activity Glycine max
3.1.3.26 34 62 34°C: about 55% of maximal activity, 62°C: about 75% of maximal activity Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60
-
 10 min, stable up to Glycine max
3.1.3.8 70
-
 10 min, 90% loss of activity Glycine max
3.1.3.8 80
-
 10 min, complete loss of activity Glycine max
3.1.3.26 60
-
 10 min, stable up to Glycine max
3.1.3.26 70
-
 10 min, 90% loss of activity Glycine max
3.1.3.26 80
-
 10 min, complete loss of activity Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4.5 5
-
 Glycine max
3.1.3.26 4.5 5
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3.8 5.8 pH 3.8: about 55% of maximal activity, pH 5.8: about 70% of maximal activity Glycine max
3.1.3.26 3.8 5.8 pH 3.8: about 55% of maximal activity, pH 5.8: about 70% of maximal activity Glycine max