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Literature summary extracted from

  • Chen, Y.R.; Hartman, F.C.; Lu, T.Y.; Larimer, F.W.
    D-Ribulose-5-phosphate 3-epimerase: cloning and heterologous expression of the spinach gene, and purification and characterization of the recombinant enzyme (1998), Plant Physiol., 118, 199-207.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.1
-
 Spinacia oleracea

General Stability

EC Number General Stability Organism
5.1.3.1 DL-alpha-glycerophosphate or ethanol stabilize the extremely labile recombinant enzyme, but are unable to reverse the spontaneous loss of activity Spinacia oleracea
5.1.3.1 drastically destabilized by 2-mercaptoethanol Spinacia oleracea
5.1.3.1 DTT destabilizes Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.1 DL-alpha-glycerophosphate competitive Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.1 0.22
-
 D-ribulose 5-phosphate 25°C, pH 8.0 Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.1 additional information no requirement for a divalent metal ion in catalysis Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.1 25000
-
 8 * 25000, SDS-PAGE Spinacia oleracea
5.1.3.1 200000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.1 Spinacia oleracea Q43157
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.1 11610
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
5.1.3.1 2°C, wether in crude extracts or highly purified, the recombinant enzyme is inherently unstable Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.1 D-Ribulose 5-phosphate
-
 Spinacia oleracea D-Xylulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.1 octamer 8 * 25000, SDS-PAGE Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
5.1.3.1 Ru5P epimerase
-
 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.3.1 0.9
-
 DL-alpha-glycerophosphate 25°C, pH 8.0 Spinacia oleracea