Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Segura, M.J.; Lodeiro, S.; Meyer, M.M.; Patel, A.J.; Matsuda, S.P.
    Directed evolution experiments reveal mutations at cycloartenol synthase residue His477 that dramatically alter catalysis (2002), Org. Lett., 4, 4459-4462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.8 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.8 H477N mutated enzyme produces lanosterol instead of cycloartenol Arabidopsis thaliana
5.4.99.8 H477Q mutated enzyme produces parkeol instead of cycloartenol Arabidopsis thaliana
5.4.99.8 Y410T/I481V mutated enzyme produces lanosterol instead of cycloartenol Arabidopsis thaliana
5.4.99.8 Y532H mutated enzyme produces a mixture of parkeol, achilleol and lanosterol instead of cycloartenol Arabidopsis thaliana
5.4.99.47 H477N the mutant produces 88% lanosterol and 12% parkeol from oxidosqualene Arabidopsis thaliana
5.4.99.47 H477Q the mutant produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol from oxidosqualene Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.8 Arabidopsis thaliana
-
-
-
5.4.99.47 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.8 (S)-2,3-Epoxysqualene
-
 Arabidopsis thaliana Cycloartenol
-
 ?
5.4.99.47 oxidosqualene
-
 Arabidopsis thaliana lanosterol + parkeol mutant H477N produces 88% lanosterol and 12% parkeol ?
5.4.99.47 oxidosqualene
-
 Arabidopsis thaliana lanosterol + parkeol mutant H477Q produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.8 AthCAS1
-
 Arabidopsis thaliana
5.4.99.47 cycloartenol synthase mutant H477N cf. EC 5.4.99.8 Arabidopsis thaliana
5.4.99.47 cycloartenol synthase mutant H477Q cf. EC 5.4.99.8 Arabidopsis thaliana