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Literature summary extracted from

  • De Angelis, M.; Curtin, A.C.; McSweeney, P.L.; Faccia, M.; Gobbetti, M.
    Lactobacillus reuteri DSM 20016: purification and characterization of a cystathionine gamma-lyase and use as adjunct starter in cheesemaking (2002), J. Dairy Res., 69, 255-267.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.4.1.1 biotechnology use of strain as adjunct starter in cheese making Limosilactobacillus reuteri

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.1 3-methyl-2-benzothiazolinone 31% residual activity at 5 mM Limosilactobacillus reuteri
4.4.1.1 DL-Penicillamine 3% residual activity at 5 mM Limosilactobacillus reuteri
4.4.1.1 DL-propargylglycine 2% residual activity at 5 mM Limosilactobacillus reuteri
4.4.1.1 iodoacetamide 22% residual activity at 2.5 mM Limosilactobacillus reuteri
4.4.1.1 additional information not inhibitory: EDTA Limosilactobacillus reuteri
4.4.1.1 N-ethylmaleimide 81% residual activity at 5 mM Limosilactobacillus reuteri
4.4.1.1 NaCl 59% residual activity at 5 mM Limosilactobacillus reuteri
4.4.1.1 phenylhydrazine 2.8% residual activity at 5 mM Limosilactobacillus reuteri
4.4.1.1 Semicarbazide 11% residual activity at 5 mM Limosilactobacillus reuteri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.1 40000
-
 4 * 40000, SDS-PAGE Limosilactobacillus reuteri
4.4.1.1 160000
-
 gel filtration Limosilactobacillus reuteri

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.1 Limosilactobacillus reuteri
-
 DSM 20016
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.1
-
 Limosilactobacillus reuteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.1 L-cystathione + H2O
-
 Limosilactobacillus reuteri L-cysteine + NH3 + 2-oxobutanoate
-
 ?
4.4.1.1 L-cysteine + H2O
-
 Limosilactobacillus reuteri sulfide + NH3 + pyruvate
-
 ?
4.4.1.1 L-cystine + H2O
-
 Limosilactobacillus reuteri NH3 + ?
-
 ?
4.4.1.1 L-djenkolic acid + H2O
-
 Limosilactobacillus reuteri NH3 + ?
-
 ?
4.4.1.1 L-methionine + H2O
-
 Limosilactobacillus reuteri NH3 + ?
-
 ?
4.4.1.1 L-serine + H2O
-
 Limosilactobacillus reuteri NH3 + ?
-
 ?
4.4.1.1 additional information activity decreases in the following order of substrates: L-cystathione, L-djenkolic, L-cystine, L-cysteine, L-methionine, L-serine Limosilactobacillus reuteri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.1 tetramer 4 * 40000, SDS-PAGE Limosilactobacillus reuteri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.1 35
-
-
 Limosilactobacillus reuteri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.1 15
-
 5 min, enzyme maintains about 50% of activity Limosilactobacillus reuteri
4.4.1.1 60
-
 5 min, complete inactivation Limosilactobacillus reuteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.1 8
-
-
 Limosilactobacillus reuteri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.1 5
-
 20% residual activity Limosilactobacillus reuteri
4.4.1.1 6
-
 40% residual activity Limosilactobacillus reuteri
4.4.1.1 9
-
 90% residual activity Limosilactobacillus reuteri

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.1 pyridoxal 5'-phosphate
-
 Limosilactobacillus reuteri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.4.1.1 Limosilactobacillus reuteri isoelectric focusing
-
 5