EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.3 | synthesis | useful in application to the processing of industrial fats and oils containing eicosapentanoic acid and docosahexaenoic acid, such as fish oil splitting | Pseudomonas fluorescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ca2+ | 1.12fold activation at 1 mM, 1.36fold activation at 3 mM, 1.51fold activation at 10 mM | Pseudomonas fluorescens | |
3.1.1.3 | K+ | slight activation | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 64000 | - |
gel filtration | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Pseudomonas fluorescens | - |
- |
- |
3.1.1.3 | Pseudomonas fluorescens HU380 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | 24.3fold | Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 9854 | - |
purified enzyme | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | methyl butyrate + H2O | - |
Pseudomonas fluorescens | methanol + butyrate | - |
? | |
3.1.1.3 | methyl butyrate + H2O | - |
Pseudomonas fluorescens HU380 | methanol + butyrate | - |
? | |
3.1.1.3 | methyl caprate + H2O | best substrate | Pseudomonas fluorescens | methanol + caprate | - |
? | |
3.1.1.3 | methyl caproate + H2O | - |
Pseudomonas fluorescens | methanol + caproate | - |
? | |
3.1.1.3 | methyl caprylate + H2O | - |
Pseudomonas fluorescens | methanol + caprylate | - |
? | |
3.1.1.3 | methyl icosanoate + H2O | - |
Pseudomonas fluorescens | methanol + icosanoate | - |
? | |
3.1.1.3 | methyl laurate + H2O | - |
Pseudomonas fluorescens | methanol + laurate | - |
? | |
3.1.1.3 | methyl linoleate + H2O | - |
Pseudomonas fluorescens | methanol + linoleate | - |
? | |
3.1.1.3 | methyl linolenate + H2O | - |
Pseudomonas fluorescens | methanol + linolenate | - |
? | |
3.1.1.3 | methyl myristate + H2O | - |
Pseudomonas fluorescens | methanol + myristate | - |
? | |
3.1.1.3 | methyl oleate + H2O | - |
Pseudomonas fluorescens | methanol + oleate | - |
? | |
3.1.1.3 | methyl palmitate + H2O | - |
Pseudomonas fluorescens | methanol + palmitate | - |
? | |
3.1.1.3 | methyl propionate + H2O | - |
Pseudomonas fluorescens | methanol + propionate | - |
? | |
3.1.1.3 | methyl propionate + H2O | - |
Pseudomonas fluorescens HU380 | methanol + propionate | - |
? | |
3.1.1.3 | methyl stearate + H2O | - |
Pseudomonas fluorescens | methanol + stearate | - |
? | |
3.1.1.3 | additional information | substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides | Pseudomonas fluorescens | ? | - |
? | |
3.1.1.3 | additional information | substrate specificity overview, enzyme splits polyunsaturated fatty acid ester bonds, the enzyme preferably acts on short- to middle-chain length fatty acid simple methyl esters and triglycerides | Pseudomonas fluorescens HU380 | ? | - |
? | |
3.1.1.3 | olive oil + H2O | emulsion | Pseudomonas fluorescens | ? | - |
? | |
3.1.1.3 | triacetin + H2O | low activity | Pseudomonas fluorescens | diacetin + acetate | - |
? | |
3.1.1.3 | tributyrin + H2O | best substrate | Pseudomonas fluorescens | dibutyrin + butyrate | - |
? | |
3.1.1.3 | tributyrin + H2O | best substrate | Pseudomonas fluorescens HU380 | dibutyrin + butyrate | - |
? | |
3.1.1.3 | tricaprin + H2O | best substrate | Pseudomonas fluorescens | dicaprin + caprate | - |
? | |
3.1.1.3 | tricaproin + H2O | best substrate | Pseudomonas fluorescens | dicaproin + caproate | - |
? | |
3.1.1.3 | tricaprylin + H2O | best substrate | Pseudomonas fluorescens | dicaprylin + caprylate | - |
? | |
3.1.1.3 | trilaurin + H2O | - |
Pseudomonas fluorescens | dilaurin + laurate | - |
? | |
3.1.1.3 | trimyristin + H2O | low activity | Pseudomonas fluorescens | dimyristin + myristate | - |
? | |
3.1.1.3 | triolein + H2O | cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond | Pseudomonas fluorescens | diolein + oleate | - |
? | |
3.1.1.3 | triolein + H2O | cleaves mainly 1,3-ester bonds, and to a lesser extent the 2-position ester bond | Pseudomonas fluorescens HU380 | diolein + oleate | - |
? | |
3.1.1.3 | tripalmitin + H2O | low activity | Pseudomonas fluorescens | dipalmitin + palmitate | - |
? | |
3.1.1.3 | tristearin + H2O | - |
Pseudomonas fluorescens | distearin + stearate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 64000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | lipase | - |
Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 45 | - |
- |
Pseudomonas fluorescens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 40 | - |
70°C, stable for 60 min, in presence of 0.1% Triton X-100 | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 8.5 | - |
- |
Pseudomonas fluorescens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.3 | Pseudomonas fluorescens | isoelectric focusing | 4.8 | 4.7 |