EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | additional information | purified rhASM can be activated in vitro by loss of the free thiol on the C-terminal cysteine via chemical modification, dimerization, or deletion of this amino acid residue | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.12 | expression in CHO cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.12 | C629S | 5fold increase in activity, modification or deletion of the thiol group on the C-terminal cysteine leads to activation of rhASM | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.12 | 1.7 | - |
2-(N-hexadecanoylamino)-4-nitrophenylphosphorylcholine | 37°C, pH 5.5 | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.12 | lysosome | - |
Homo sapiens | 5764 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Cu2+ | 6fold increase in activity at 0.1 mM | Homo sapiens | |
3.1.4.12 | Zn2+ | 5fold increase in activity at 0.1 mM | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Homo sapiens | - |
one form of Niemann-Pick disease is caused by a deficiency of acid sphingomyelinase | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.4.12 | glycoprotein | at least five of six potential glycosylation sites are utilized | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 21.1 | - |
- |
Homo sapiens |
3.1.4.12 | 92.8 | - |
5fold higher specific activity of rhASM purified from CHO cell-harvests stored at -20°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | 2-(N-hexadecanoylamino)-4-nitrophenylphosphorylcholine + H2O | - |
Homo sapiens | 2-(N-hexadecanoylamino)-4-nitrophenol + choline phosphate | - |
? | |
3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | acid sphingomyelinase | - |
Homo sapiens |
3.1.4.12 | rhASM | - |
Homo sapiens |