EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.5 | gene ileS2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strains DH5alpha and Ts331 mediates resistance against pseudomonic acid, complementation of the ileS-deficient strain Ts331 | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.5 | additional information | no inhibition of isozyme IleRS-R2 by pseudomonic acid, i.e. muciproin | Pseudomonas fluorescens | |
6.1.1.5 | pseudomonic acid | i.e. muciproin | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.5 | 0.00125 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
6.1.1.5 | 0.007 | - |
ATP | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
6.1.1.5 | 0.01 | - |
ATP | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
6.1.1.5 | 0.012 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
6.1.1.5 | 0.285 | - |
ATP | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
6.1.1.5 | 0.33 | - |
ATP | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.5 | ATP + L-isoleucine + tRNAIle | Pseudomonas fluorescens | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.1.1.5 | ATP + L-isoleucine + tRNAIle | Pseudomonas fluorescens NCIB 10586 | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.5 | Pseudomonas fluorescens | - |
gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli | - |
6.1.1.5 | Pseudomonas fluorescens | Q8L1B1 | pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2 | - |
6.1.1.5 | Pseudomonas fluorescens NCIB 10586 | - |
gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli | - |
6.1.1.5 | Pseudomonas fluorescens NCIB 10586 | Q8L1B1 | pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.5 | native enzyme, and recombinant enzyme from Escherichia coli, amino acid sequence determination | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.5 | ATP + L-isoleucine + tRNAIle | - |
Pseudomonas fluorescens | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.1.1.5 | ATP + L-isoleucine + tRNAIle | tRNAIle from Pseudomonas fluorescens and Escherichia coli | Pseudomonas fluorescens | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.1.1.5 | ATP + L-isoleucine + tRNAIle | - |
Pseudomonas fluorescens NCIB 10586 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
6.1.1.5 | ATP + L-isoleucine + tRNAIle | tRNAIle from Pseudomonas fluorescens and Escherichia coli | Pseudomonas fluorescens NCIB 10586 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.5 | ? | x * 117000-117738, native isozyme IleRS-R2, SDS-PAGE and amino acid sequence determination | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.5 | IleRS | - |
Pseudomonas fluorescens |
6.1.1.5 | IRS | - |
Pseudomonas fluorescens |
6.1.1.5 | Isoleucine translase | - |
Pseudomonas fluorescens |
6.1.1.5 | Isoleucine--tRNA ligase | - |
Pseudomonas fluorescens |
6.1.1.5 | Isoleucine-transfer RNA ligase | - |
Pseudomonas fluorescens |
6.1.1.5 | Isoleucine-tRNA synthetase | - |
Pseudomonas fluorescens |
6.1.1.5 | Isoleucyl-transfer ribonucleate synthetase | - |
Pseudomonas fluorescens |
6.1.1.5 | Isoleucyl-transfer RNA synthetase | - |
Pseudomonas fluorescens |
6.1.1.5 | Isoleucyl-tRNA synthetase | - |
Pseudomonas fluorescens |
6.1.1.5 | Mupirocin resistance protein | - |
Pseudomonas fluorescens |
6.1.1.5 | Synthetase, isoleucyl-transfer ribonucleate | - |
Pseudomonas fluorescens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.5 | 0.18 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
6.1.1.5 | 2.64 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
6.1.1.5 | 3 | 6 | L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
6.1.1.5 | 3.3 | - |
L-isoleucine | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
6.1.1.5 | 4.63 | - |
L-isoleucine | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.5 | 7.5 | - |
assay at | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.5 | ATP | - |
Pseudomonas fluorescens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.5 | 0.0000105 | - |
pseudomonic acid | pH 7.5, recombinant isozyme IleRS-R1, versus L-isoleucine | Pseudomonas fluorescens | |
6.1.1.5 | 0.000015 | - |
pseudomonic acid | pH 7.5, recombinant isozyme IleRS-R1, versus ATP | Pseudomonas fluorescens |