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Literature summary extracted from

  • Kesarwani, M.; Azam, M.; Natarajan, K.; Mehta, A.; Datta, A.
    Oxalate decarboxylase from Collybia velutipes. Molecular cloning and its overexpression to confer resistance to fungal infection in transgenic tobacco and tomato (2000), J. Biol. Chem., 275, 7230-7238.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.2 agriculture development of transgenic plants resistant to fungal infection, transgenic tobacco and tomato plants expressing oxalate decarboxylase show remarkable resistance to phytopathogenic fungus Sclerotinia sclerotiorum that utilizes oxalic acid during infestation Flammulina velutipes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.2 expression of functionally inactive OXDC in Escherichia coli, expression of active OXDC in Nicotiana tabaccum var. Petite Havanna and tomato plants var. Pusa Ruby in cytosol and vacuole Flammulina velutipes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.2 4.5
-
 oxalate native OXDC Flammulina velutipes
4.1.1.2 5
-
 oxalate recombinant OXDC, expressed in Nicotiana tabaccum Flammulina velutipes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.2 50000
-
 x * 50000, deglycosylated form, calculated from the amino acid sequence Flammulina velutipes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 oxalate + H+ Flammulina velutipes oxalate-catabolizing enzyme formate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Flammulina velutipes Q9UVK4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.2 glycoprotein recombinant enzyme, expressed in tobacco or tomato plants, is partially glycosylated, four potential N-linked glycosylation sites Flammulina velutipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+ specific for oxalate Flammulina velutipes formate + CO2
-
 ?
4.1.1.2 oxalate + H+ oxalate-catabolizing enzyme Flammulina velutipes formate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.2 ? x * 50000, deglycosylated form, calculated from the amino acid sequence Flammulina velutipes

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 OXDC
-
 Flammulina velutipes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.2 additional information
-
 active at low pH Flammulina velutipes

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.2 additional information no cofactor requirement Flammulina velutipes