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Literature summary extracted from

  • Araki, H.; Li, Y.; Yamamoto, Y.; Haneda, M.; Nishi, K.; Kikkawa, R.; Ohkubo, I.
    Purification, molecular cloning, and immunohistochemical localization of dipeptidyl peptidase II from the rat kidney and its identity with quiescent cell proline dipeptidase (2001), J. Biochem., 129, 279-288.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.9 DNA and amino acid sequence analysis, high homology to dipeptidyl-peptidase II, EC 3.4.14.2 Homo sapiens
3.4.14.2 DNA sequence determination and analysis, sequence contains a leucine zipper motif Rattus norvegicus

General Stability

EC Number General Stability Organism
3.4.14.2 enzyme is stable during several repeated cycles of freezing and thawing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.2 4-(2-aminoethyl)benzenesulfonyl fluoride 60% inhibition at 1 mM, preincubation at room temperature for 15 min Rattus norvegicus
3.4.14.2 benzamidine 10% inhibition at 1 mM, preincubation at room temperature for 15 min Rattus norvegicus
3.4.14.2 Cu2+ 40% inhibition at 1 mM, preincubation at room temperature for 15 min Rattus norvegicus
3.4.14.2 diisopropylfluorophosphate complete inhibition at 1 mM, 25% inhibition at 0.1 mM, preincubation at room temperature for 15 min Rattus norvegicus
3.4.14.2 Hg2+ complete inhibition at 1 mM, preincubation at room temperature for 15 min Rattus norvegicus
3.4.14.2 additional information poor or no inhibition by EDTA, NEM, E-64, diprotin A, and actinonin Rattus norvegicus
3.4.14.2 PCMBS 77% inhibition at 1 mM, preincubation at room temperature for 15 min Rattus norvegicus
3.4.14.2 PMSF 45% inhibition at 1 mM, preincubation at room temperature for 15 min Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.2 lysosome
-
 Rattus norvegicus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.9 58000
-
 2 * 58000 Homo sapiens
3.4.14.2 54054
-
 2 * 54054, mass spectrometry Rattus norvegicus
3.4.14.2 105000
-
 gel filtration and native PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Homo sapiens
-
-
-
3.4.14.2 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.13.9 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.9 to homogeneity Homo sapiens
3.4.14.2 131.3fold to homogeneity, from kidney Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.9 liver
-
 Homo sapiens
-
3.4.13.9 T-cell
-
 Homo sapiens
-
3.4.14.2 aorta
-
 Rattus norvegicus
-
3.4.14.2 brain
-
 Rattus norvegicus
-
3.4.14.2 cerebellum
-
 Rattus norvegicus
-
3.4.14.2 cerebrum
-
 Rattus norvegicus
-
3.4.14.2 epididymis
-
 Rattus norvegicus
-
3.4.14.2 esophagus
-
 Rattus norvegicus
-
3.4.14.2 heart
-
 Rattus norvegicus
-
3.4.14.2 kidney
-
 Rattus norvegicus
-
3.4.14.2 large intestine
-
 Rattus norvegicus
-
3.4.14.2 liver
-
 Rattus norvegicus
-
3.4.14.2 lung
-
 Rattus norvegicus
-
3.4.14.2 additional information tissue distribution, overview Rattus norvegicus
-
3.4.14.2 muscle
-
 Rattus norvegicus
-
3.4.14.2 pancreas
-
 Rattus norvegicus
-
3.4.14.2 prostate
-
 Rattus norvegicus
-
3.4.14.2 skeletal muscle
-
 Rattus norvegicus
-
3.4.14.2 small intestine
-
 Rattus norvegicus
-
3.4.14.2 spermatozoon
-
 Rattus norvegicus
-
3.4.14.2 spleen
-
 Rattus norvegicus
-
3.4.14.2 stomach
-
 Rattus norvegicus
-
3.4.14.2 testis
-
 Rattus norvegicus
-
3.4.14.2 thymus
-
 Rattus norvegicus
-
3.4.14.2 trachea
-
 Rattus norvegicus
-
3.4.14.2 urinary bladder
-
 Rattus norvegicus
-
3.4.14.2 uterus
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.2 0.3
-
 purified enzyme, substrate Ala-Ala-2-nitroanilide Rattus norvegicus
3.4.14.2 1.05
-
 purified enzyme, substrate Lys-Ala-7-amido-4-methylcoumarin Rattus norvegicus
3.4.14.2 2.11
-
 purified enzyme, substrate Gly-Pro-7-amido-4-methylcoumarin Rattus norvegicus
3.4.14.2 2.85
-
 purified enzyme, substrate Ala-Pro-amino-4-trifluoromethylcoumarin Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.14.2 -30°C, 20 mM sodium phosphate-buffered saline, pH 8.0, stable for at least several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 Ala-Pro-amino-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens ?
-
 ?
3.4.14.2 Ala-Ala-2-nitroanilide + H2O low activity Rattus norvegicus Ala-Ala + 2-nitroaniline
-
 ?
3.4.14.2 Ala-Pro-amino-4-trifluoromethylcoumarin + H2O
-
 Rattus norvegicus Ala-Pro + amino-4-trifluoromethylcoumarin
-
 ?
3.4.14.2 Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.2 Lys-Ala-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus Lys-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.14.2 additional information substrate specificity Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.9 dimer 2 * 58000 Homo sapiens
3.4.13.9 More enzyme contains a leucine zipper motif Homo sapiens
3.4.14.2 dimer 2 * 54054, mass spectrometry Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.13.9 More enzyme belongs to the methionyl amino peptidase family M24, fomerly EC 3.4.3.7 Homo sapiens
3.4.13.9 QPP
-
 Homo sapiens
3.4.13.9 quiescent cell proline dipeptidase
-
 Homo sapiens
3.4.14.2 More enzyme belongs to the Pro-X carboxypeptidase family S28 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.9 37
-
 assay at Homo sapiens
3.4.14.2 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.2 50
-
 30 min, stable up to Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.9 6
-
 assay at Homo sapiens
3.4.14.2 5.5
-
 with substrate Lys-Ala-7-amido-4-methylcoumarin Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.2 2.5 11.5
-
 Rattus norvegicus