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Literature summary extracted from
- Mechanism of chloride elimination from 3-chloro- and 2,4-dichloro-cis,cis-muconate: new insight obtained from analysis of muconate cycloisomerase variant CatB-K169A (2001), J. Bacteriol., 183, 4551-4561.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.5.1.1 | - |
Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.5.1.1 | K169A | 70000fold reduction in activity with cis,cis-muconate, 110000fold reduction in activity with 3-fluoro-cis,cis-muconate, 310fold reduction in activity with 3-chloro-cis,cis-muconate, 2.6fold reduction in activity with 2,4-dichloro-cis,cis-muconate. Wild-type enzyme forms protoanemonin and 2-chloroprotoanemonin from 3-chloro-cis,cis-muconate, mutant enzyme K169A forms cis-dienelactone and 2-chloro-cis-dienelactone | Pseudomonas putida |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.5.1.1 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.5.1.1 | recombinant enzyme | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.1 | 2,4-dichloro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? |  |
| 5.5.1.1 | 3-chloro-cis,cis-muconate | - |
Pseudomonas putida | additional information | wild-type enzyme forms protoanemonin and 2-chloroprotoanemonin, mutant enzyme K169A forms cis-dienelactone and 2-chloro-cis-dienelactone | ? | |
| 5.5.1.1 | 3-Fluoro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
| 5.5.1.1 | cis,cis-muconate | - |
Pseudomonas putida | muconolactone | - |
? | |
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Release 2023.1 (January 2023)
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