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Literature summary extracted from

  • Kaczmarek, F.S.; Zaniewski, R.P.; Gootz, T.D.; Danley, D.E.; Mansour, M.N.; Griffor, M.; Kamath, A.V.; Cronan, M.; Mueller, J.; Sun, D.; Martin, P.K.; Benton, B.; McDowell, L.; Biek, D.; Schmid, M.B.
    Cloning and functional characterization of an NAD(+)-dependent DNA ligase from Staphylococcus aureus (2001), J. Bacteriol., 183, 3016-3024.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 wild type and fragments expressed in Escherichia coli GI724 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.2 DELTA1-390 DNA binding, but no self adenylation activity Staphylococcus aureus
6.5.1.2 DELTA316-667 active in self adenylation but unable to bind DNA Staphylococcus aureus
6.5.1.2 additional information no ligation activity when both fragments are mixed together Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.2 75290
-
 mass spectrometry Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Staphylococcus aureus DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 recombinant proteins from Escherichia coli Staphylococcus aureus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.2 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Staphylococcus aureus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Staphylococcus aureus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Staphylococcus aureus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.2 8.5
-
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+ required, highest activity at 0.03 mM Staphylococcus aureus