Any feedback?
Literature summary extracted from
- Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase (2001), J. Bacteriol., 183, 2226-2233.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.1 | expression in Escherichia coli. Heterologous expression renders Saccharomyces cerevisiae capable of utilization of D-Ala as a nitrogen source but also relieves the yeast from the toxicity of D-Ala | Schizosaccharomyces pombe |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.1 | Sodium borohydride | complete inactivation after dialysis against | Schizosaccharomyces pombe |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.1 | 2.4 | - |
D-Ala | 37°C, pH 9.0 | Schizosaccharomyces pombe | |
| 5.1.1.1 | 5 | - |
L-Ala | 37°C, pH 9.0 | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 44000 | - |
gel filtration | Schizosaccharomyces pombe |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.1 | Schizosaccharomyces pombe | O59828 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.1 | recombinant enzyme | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.1 | L-2-Aminobutyrate | 0.37% of the activity with L-Ala | Schizosaccharomyces pombe | D-2-Aminobutyrate | - |
r | |
| 5.1.1.1 | L-Ala | - |
Schizosaccharomyces pombe | D-Ala | - |
r | |
| 5.1.1.1 | L-Ser | 3.7% of the activity with L-Ala | Schizosaccharomyces pombe | D-Ser | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.1 | monomer | 1 * 44000, SDS-PAGE | Schizosaccharomyces pombe |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.1 | 9 | - |
D-Ala as substrate | Schizosaccharomyces pombe |
| 5.1.1.1 | 9.5 | - |
L-Ala as substrate | Schizosaccharomyces pombe |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.1 | pyridoxal 5'-phosphate | coenzyme | Schizosaccharomyces pombe | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
