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Literature summary extracted from

  • Tanner, A.; Bornemann, S.
    Bacillus subtilis YvrK is an acid-induced oxalate decarboxylase (2000), J. Bacteriol., 182, 5271-5273.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.2 additional information pH-dependent induction in acidic growth media, particularly at pH 5, but not by oxalate Bacillus subtilis
4.1.1.2 O2 dioxygen-dependent reaction suggesting the role of a transition metal in catalysis Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.2 cytosol
-
 Bacillus subtilis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.2 additional information presence of a metal ion, role of a transition metal in catalysis Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.2 43407
-
 5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer Bacillus subtilis
4.1.1.2 44000
-
 5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer Bacillus subtilis
4.1.1.2 220000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 oxalate + H+ Bacillus subtilis oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation formate + CO2
-
 ?
4.1.1.2 oxalate + H+ Bacillus subtilis 168 oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation formate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Bacillus subtilis
-
-
-
4.1.1.2 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.2 1000fold Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.2 additional information
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
4.1.1.2 -20°C, frozen in liquid nitrogen, pH buffered at or near 7, stable Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+ acts exclusively on oxalate Bacillus subtilis formate + CO2
-
 ?
4.1.1.2 oxalate + H+ oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation Bacillus subtilis formate + CO2
-
 ?
4.1.1.2 oxalate + H+ acts exclusively on oxalate Bacillus subtilis 168 formate + CO2
-
 ?
4.1.1.2 oxalate + H+ oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation Bacillus subtilis 168 formate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.2 pentamer 5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 More belongs to the cupin superfamily, bicupin Bacillus subtilis
4.1.1.2 YvrK
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.2 5
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.2 3 7.5 70% of maximum activity at pH 3, no activity at pH 7.5 Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.2 Bacillus subtilis predicted value
-
 5.1
4.1.1.2 Bacillus subtilis determined using PhastSystem
-
 6.1