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Literature summary extracted from

  • Kim, S.W.; Choi, K.Y.
    Identification of active site residues by site-directed mutagenesis of DELTA5-3-ketosteroid isomerase from Pseudomonas putida biotype B (1995), J. Bacteriol., 177, 2602-2605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.1 expression of mutant enzymes D40N and Y16F in Escherichia col BL21 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.1 D40N 1484555fold decrease in turnover number, 4.46fold decrease in KM-value as compared to wild-type enzyme Pseudomonas putida
5.3.3.1 Y16F 2009fold decrease in turnover number, 3.5fold decrease in KM-value as compared to wild-type enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.1 additional information
-
 additional information Km-values of mutant enzymes Y16F and D40N Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.1 14520
-
 mutant enzyme D40N, electrospray mass spectrometry Pseudomonas putida
5.3.3.1 14520
-
 mutant enzyme Y16F, electrospray mass spectrometry Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.1 Pseudomonas putida
-
 biotype B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.1 mutant enzymes D40N and Y16F Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.1 additional information
-
 additional information turnover number of mutant enzymes Y16F and D40N Pseudomonas putida