EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.13 | DL-homocysteine | 12.5 mM, 70% inhibition, competitive inhibitor | Brassica oleracea | |
4.4.1.13 | hydroxylamine | 12.5 mM, 97% inhibition | Brassica oleracea | |
4.4.1.13 | L-cysteine | 12 mM, 65% inhibition after 5 min, 1 mM, 29% inhibition after 10 min, linear noncompetitive inhibitor | Brassica oleracea | |
4.4.1.13 | NEM | 1.25 mM, about 45% inhibition | Brassica oleracea | |
4.4.1.13 | pyridoxal | 12.5 mM, in presence of 12 mM L-cystine 12% inhibition, in presence of 1 mM L-cystine 37% inhibition | Brassica oleracea | |
4.4.1.13 | pyruvate | 12.5 mM, 25% inhibition | Brassica oleracea | |
4.4.1.13 | Sodium cyanide | 12.5 mM, 95% inhibition | Brassica oleracea | |
4.4.1.35 | cyanide | 12.5 mM, 5% residual activity | Brassica oleracea var. italica | |
4.4.1.35 | DL-homocysteine | 2.5 mM, 59% residual activity | Brassica oleracea var. italica | |
4.4.1.35 | hydroxylamine | 12.5 mM, 3% residual activity | Brassica oleracea var. italica | |
4.4.1.35 | L-cysteine | linear noncompetitive inhibitor | Brassica oleracea var. italica | |
4.4.1.35 | pyridoxal | 2.5 mM, 63% residual activity | Brassica oleracea var. italica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.13 | 0.0812 | - |
L-cystine | - |
Brassica oleracea | |
4.4.1.35 | 0.0812 | - |
L-cystine | pH 8.4, 30°C | Brassica oleracea var. italica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.4.1.13 | 48000 | - |
2 * 48000, SDS-PAGE | Brassica oleracea |
4.4.1.13 | 100000 | - |
gel filtration | Brassica oleracea |
4.4.1.35 | 47000 | - |
- |
Brassica oleracea var. italica |
4.4.1.35 | 48000 | - |
- |
Brassica oleracea var. italica |
4.4.1.35 | 94000 | - |
MALDI-TOF | Brassica oleracea var. italica |
4.4.1.35 | 100000 | 110000 | gel filtration | Brassica oleracea var. italica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.13 | additional information | Brassica oleracea | the enzyme is responsible for off-aroma deterioration in fresh unblanched broccoli | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.13 | Brassica oleracea | - |
var. italica | - |
4.4.1.35 | Brassica oleracea var. italica | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
4.4.1.13 | glycoprotein | carbohydrate content of 3% | Brassica oleracea |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.13 | - |
Brassica oleracea |
4.4.1.35 | - |
Brassica oleracea var. italica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.4.1.13 | 7.69 | - |
- |
Brassica oleracea |
4.4.1.35 | 7.7 | - |
pH 8.4, 30°C | Brassica oleracea var. italica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.13 | cystathionine + H2O | 1-4% of the activity with L-cysteine, depending on assay method | Brassica oleracea | L-homocysteine + NH3 + pyruvate | - |
? | |
4.4.1.13 | D-cystine + H2O | 2% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
4.4.1.13 | L-cysteine S-sulfate + H2O | 11% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
4.4.1.13 | L-cystine + H2O | - |
Brassica oleracea | L-thiocysteine + pyruvate + NH3 | - |
? | |
4.4.1.13 | L-djenkolic acid + H2O | 18-24% of the activity with L-cystine, depending on assay method | Brassica oleracea | ? | - |
? | |
4.4.1.13 | additional information | the enzyme is responsible for off-aroma deterioration in fresh unblanched broccoli | Brassica oleracea | ? | - |
? | |
4.4.1.13 | S-ethyl-L-cysteine + H2O | 18% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
4.4.1.13 | S-ethyl-L-cysteine sulfoxide + H2O | 71% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
4.4.1.13 | S-methyl-L-cysteine + H2O | 13-29% of the activity with L-cystine, depending on assay method | Brassica oleracea | ? | - |
? | |
4.4.1.13 | S-propyl-L-cysteine + H2O | 29% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
4.4.1.35 | L-cysteine S-sulfate + H2O | - |
Brassica oleracea var. italica | ? | - |
? | |
4.4.1.35 | L-cystine + H2O | - |
Brassica oleracea var. italica | L-thiocysteine + pyruvate + NH3 | overall reaction | ? | |
4.4.1.35 | L-djenkolic acid + H2O | 18-24% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? | |
4.4.1.35 | additional information | negligible activity with L-cystathionine | Brassica oleracea var. italica | ? | - |
? | |
4.4.1.35 | S-ethyl L-cysteine + H2O | 71% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? | |
4.4.1.35 | S-methyl L-cysteine + H2O | 29% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? | |
4.4.1.35 | S-propyl L-cysteine + H2O | 29% of the activity with L-cystine | Brassica oleracea var. italica | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.13 | dimer | 2 * 48000, SDS-PAGE | Brassica oleracea |
4.4.1.35 | dimer | 2 * 47000, MALDI-TOF, 2 * 48000, SDS-PAGE | Brassica oleracea var. italica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.13 | 35 | - |
- |
Brassica oleracea |
4.4.1.35 | 35 | - |
- |
Brassica oleracea var. italica |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.13 | 45 | - |
10 min, completely stable up to | Brassica oleracea |
4.4.1.13 | 50 | - |
10 min, 20% loss of activity | Brassica oleracea |
4.4.1.13 | 60 | - |
10 min, 75% loss of actrivity | Brassica oleracea |
4.4.1.35 | 45 | - |
10 min, stable | Brassica oleracea var. italica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.13 | 9.1 | - |
- |
Brassica oleracea |
4.4.1.35 | 9.1 | - |
- |
Brassica oleracea var. italica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.13 | 8.4 | 9.9 | pH 8.4: about 55% of maximal activity, pH 9.9: about 30% of maximal activity | Brassica oleracea |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.35 | 3 | - |
complete loss of activity | Brassica oleracea var. italica |
4.4.1.35 | 4 | 8 | 30°C, stable for 24 h | Brassica oleracea var. italica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.13 | pyridoxal 5'-phosphate | contains 2 mol per mol of enzyme | Brassica oleracea | |
4.4.1.35 | pyridoxal 5'-phosphate | - |
Brassica oleracea var. italica |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.13 | 1.5 | - |
DL-homocysteine | - |
Brassica oleracea | |
4.4.1.13 | 5 | - |
L-cysteine | - |
Brassica oleracea | |
4.4.1.35 | 1.5 | - |
L-cysteine | pH 8.4, 30°C | Brassica oleracea var. italica |