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Literature summary extracted from

  • Zuniga, R.; Salazar, J.; Canales, M.; Orellana, O.
    A dispensable peptide from Acidithiobacillus ferrooxidans tryptophanyl-tRNA synthetase affects tRNA binding (2002), FEBS Lett., 532, 387-390.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.2 DNA sequence analysis, expression of wild-type enzyme and a mutant enzyme, truncated in the CP2 domain, in Escherichia coli DH5alpha as fusion proteins with glutathione S-transferase Acidithiobacillus ferrooxidans
6.1.1.2 expression in strain DH5alpha Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.2 0.00031
-
 tRNATrp recombinant wild-type Acidithiobacillus ferrooxidans
6.1.1.2 0.00062
-
 tRNATrp recombinant mutant Escherichia coli
6.1.1.2 0.0015
-
 L-tryptophan recombinant wild-type Acidithiobacillus ferrooxidans
6.1.1.2 0.0019
-
 L-tryptophan recombinant truncated mutant Acidithiobacillus ferrooxidans
6.1.1.2 0.002
-
 tRNATrp recombinant truncated mutant Acidithiobacillus ferrooxidans
6.1.1.2 0.017
-
 L-tryptophan recombinant enzyme Escherichia coli
6.1.1.2 0.129
-
 ATP recombinant wild-type Acidithiobacillus ferrooxidans
6.1.1.2 0.252
-
 ATP recombinant truncated mutant Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp Acidithiobacillus ferrooxidans
-
 AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp Escherichia coli
-
 AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Acidithiobacillus ferrooxidans
-
 gene trpS
-
6.1.1.2 Escherichia coli
-
 gene trpS
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
 Acidithiobacillus ferrooxidans AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?
6.1.1.2 ATP + L-tryptophan + tRNATrp
-
 Escherichia coli AMP + diphosphate + L-tryptophan-tRNATrp
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.2 TrpRS
-
 Acidithiobacillus ferrooxidans
6.1.1.2 TrpRS
-
 Escherichia coli
6.1.1.2 Tryptophanyl-tRNA synthase
-
 Acidithiobacillus ferrooxidans
6.1.1.2 Tryptophanyl-tRNA synthase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.2 2.22
-
 tRNATrp recombinant truncated mutant Acidithiobacillus ferrooxidans
6.1.1.2 2.94
-
 tRNATrp recombinant truncated mutant Acidithiobacillus ferrooxidans
6.1.1.2 3 6 tRNATrp recombinant wild-type Acidithiobacillus ferrooxidans
6.1.1.2 3.17
-
 tRNATrp recombinant wild-type Acidithiobacillus ferrooxidans
6.1.1.2 13.6
-
 L-tryptophan recombinant truncated mutant Acidithiobacillus ferrooxidans
6.1.1.2 13.9
-
 tRNATrp recombinant mutant Escherichia coli
6.1.1.2 24.5
-
 L-tryptophan recombinant wild-type Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.2 ATP
-
 Acidithiobacillus ferrooxidans
6.1.1.2 ATP
-
 Escherichia coli