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Literature summary extracted from

  • Esposito, G.; Vitagliano, L.; Santamaria, R.; Viola, A.; Zagari, A.; Salvatore, F.
    Structural and functional analysis of aldolase B mutants related to hereditary fructose intolerance (2002), FEBS Lett., 531, 152-156.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.13 medicine aldolase B deficiency causes hereditary fructose intolerance Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.13 A149P no catalytic activity Homo sapiens
4.1.2.13 L256P melting temperature is 40°C versus 47°C for the wild-type enzyme Homo sapiens
4.1.2.13 N334K increased Km value Homo sapiens
4.1.2.13 Q354E kinetis resemble that of the wild-type enzyme Homo sapiens
4.1.2.13 W147R melting temperature is 40°C versus 47°C for the wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.0017
-
 D-Fructose 1-phosphate W147R mutant, 22°C Homo sapiens
4.1.2.13 0.00173
-
 D-Fructose 1-phosphate L256P mutant, 22°C Homo sapiens
4.1.2.13 0.0022
-
 D-Fructose 1-phosphate wild-type enzyme, 22°C Homo sapiens
4.1.2.13 0.0023
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 22°C Homo sapiens
4.1.2.13 0.0055
-
 D-fructose 1,6-bisphosphate W147R mutant, 22°C Homo sapiens
4.1.2.13 0.0073
-
 D-fructose 1,6-bisphosphate L256P mutant, 22°C Homo sapiens
4.1.2.13 0.0091
-
 D-fructose 1,6-bisphosphate Q354E mutant, 22°C Homo sapiens
4.1.2.13 0.022
-
 D-Fructose 1-phosphate N334K mutant, 22°C Homo sapiens
4.1.2.13 0.032
-
 D-fructose 1,6-bisphosphate N334K mutant, 22°C Homo sapiens
4.1.2.13 0.05
-
 D-Fructose 1-phosphate Q354E mutant, 22°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 170000
-
 tetrameric form, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate Homo sapiens
-
 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Homo sapiens P05062
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13 95% homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.13 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.13 0.13
-
 N334K mutant, D-fructose 1-phosphate as substrate Homo sapiens
4.1.2.13 0.28
-
 N334K mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
4.1.2.13 0.38
-
 W147R mutant, D-fructose 1-phosphate as substrate Homo sapiens
4.1.2.13 0.58
-
 L256P mutant, D-fructose 1-phosphate as substrate Homo sapiens
4.1.2.13 0.64
-
 W147R mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
4.1.2.13 0.81
-
 L256P mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
4.1.2.13 0.86
-
 Q354E mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
4.1.2.13 0.88
-
 Q354E mutant, D-fructose 1-phosphate as substrate Homo sapiens
4.1.2.13 0.94
-
 wild-type enzyme, D-fructose 1-phosphate as substrate Homo sapiens
4.1.2.13 0.97
-
 wild-type enzyme, D-fructose 1,6-bisphosphate as substrate Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.1.2.13 -20°C, wild-type and Q354E mutant: stable in 50% glycerol for 1 month, W147R and L256P mutant enzyme: aggregate after 15 days suggesting structural instability Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Homo sapiens glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
4.1.2.13 D-Fructose 1-phosphate
-
 Homo sapiens Glycerone phosphate + D-glyceraldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.13 More A149P mutant shows tetrameric and monomeric forms and other species with molecular masses intermediate between those corresponding to the tetramer and monomer Homo sapiens
4.1.2.13 tetramer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.13 aldolase B
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.13 40
-
 W147R melting temperature of W147R mutant and L256P mutant is 40°C versus 47°C for the wild-type enzyme Homo sapiens
4.1.2.13 47
-
 melting temperature of the wild-type enzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.13 0.059
-
 D-Fructose 1-phosphate Q354E mutant, 22°C Homo sapiens
4.1.2.13 0.085
-
 D-Fructose 1-phosphate N334K mutant, 22°C Homo sapiens
4.1.2.13 0.19
-
 D-fructose 1,6-bisphosphate N334K mutant, 22°C Homo sapiens
4.1.2.13 0.25
-
 D-Fructose 1-phosphate W147R mutant, 22°C Homo sapiens
4.1.2.13 0.38
-
 D-Fructose 1-phosphate L256P mutant, 22°C Homo sapiens
4.1.2.13 0.43
-
 D-fructose 1,6-bisphosphate W147R mutant, 22°C Homo sapiens
4.1.2.13 0.54
-
 D-fructose 1,6-bisphosphate L256P mutant, 22°C Homo sapiens
4.1.2.13 0.57
-
 D-fructose 1,6-bisphosphate Q354E mutant, 22°C Homo sapiens
4.1.2.13 0.61
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 22°C Homo sapiens
4.1.2.13 0.67
-
 D-Fructose 1-phosphate wild-type enzyme, 22°C Homo sapiens
4.1.2.13 6.08
-
 D-fructose 1,6-bisphosphate L256P mutant, 22°C Homo sapiens
4.1.2.13 6.08
-
 D-fructose 1,6-bisphosphate Q354E mutant, 22°C Homo sapiens
4.1.2.13 6.08
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 22°C Homo sapiens
4.1.2.13 6.08
-
 D-Fructose 1-phosphate wild-type enzyme, 22°C Homo sapiens