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Literature summary extracted from

  • Evans, L.D.B.; Roszak, A.W.; Noble, L.J.; Robinson, D.A.; Chalk, P.A.; Matthews, J.L.; Coggins, J.R.; Price, N.C.; Lapthorn, A.J.
    Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions (2002), FEBS Lett., 530, 24-30.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.10 expressed in Escherichia coli Streptomyces coelicolor
4.2.1.10 expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.10 hanging drop vapor diffusion method Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.10 phosphate
-
 Streptomyces coelicolor
4.2.1.10 sulfate
-
 Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.10 0.024
-
 3-dehydroquinate pH 7, 25°C Mycobacterium tuberculosis
4.2.1.10 0.1
-
 3-dehydroquinate pH 7, 25°C Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Mycobacterium tuberculosis P9WPX7
-
-
4.2.1.10 Mycobacterium tuberculosis H37Rv P9WPX7
-
-
4.2.1.10 Streptomyces coelicolor
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.10 3-dehydroquinate = 3-dehydroshikimate + H2O type I and type II DHQases are quite distinct from one another in terms of amino acid sequences and three-dimensional structures. Type I enzymes involve a syn elimination and catalyse the biosynthetic reaction. By contrast type II enzymes, which can catalyse either the biosynthetic or catabolic reactions, involve and anti elimination reaction Streptomyces coelicolor
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 3-dehydroquinate
-
 Streptomyces coelicolor 3-dehydroshikimate + H2O
-
 ?
4.2.1.10 3-dehydroquinate
-
 Mycobacterium tuberculosis 3-dehydroshikimate + H2O
-
 ?
4.2.1.10 3-dehydroquinate
-
 Mycobacterium tuberculosis H37Rv 3-dehydroshikimate + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.10 5.2
-
 3-dehydroquinate pH 7, 25°C Mycobacterium tuberculosis
4.2.1.10 124
-
 3-dehydroquinate pH 7, 25°C Streptomyces coelicolor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.10 7
-
 phosphate pH 7, 25°C Streptomyces coelicolor
4.2.1.10 11
-
 sulfate pH 7, 25°C Streptomyces coelicolor