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Literature summary extracted from

  • Takao, M.; Nakaniwa, T.; Yoshikawa, K.; Terashita, T.; Sakai, T.
    Purification and characterization of thermostable pectate lyase with protopectinase activity from thermophilic Bacillus sp. TS 47 (2000), Biosci. Biotechnol. Biochem., 64, 2360-2367.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Ag+ 1 mM, strong Bacillus sp. (in: Bacteria)
4.2.2.2 Ba2+ 1 mM, strong Bacillus sp. (in: Bacteria)
4.2.2.2 Cd2+ 1 mM, moderate Bacillus sp. (in: Bacteria)
4.2.2.2 Cu2+ 1 mM, moderate Bacillus sp. (in: Bacteria)
4.2.2.2 EDTA activity is recovered by addition of Ca2+ Bacillus sp. (in: Bacteria)
4.2.2.2 Mn2+ 1 mM, moderate Bacillus sp. (in: Bacteria)
4.2.2.2 Zn2+ 1 mM, strong Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information Km-value for polygalacturonic acid is 0.85 mg/ml Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.2 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ required Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 50000
-
 x * 50000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Bacillus sp. (in: Bacteria)
-
 TS 47
-
4.2.2.2 Bacillus sp. (in: Bacteria) TS 47
-
 TS 47
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.2 additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 pectin
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
4.2.2.2 pectin
-
 Bacillus sp. (in: Bacteria) TS 47 ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) unsaturated galacturonate
-
 ?
4.2.2.2 polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) TS 47 unsaturated galacturonate
-
 ?
4.2.2.2 protopectin
-
 Bacillus sp. (in: Bacteria) pectin + ?
-
 ?
4.2.2.2 protopectin
-
 Bacillus sp. (in: Bacteria) TS 47 pectin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 50000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 endo-polygalacturonate trans-eliminase
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 PL
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 70
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 60 75 50°C: about 20% of maximal activity, 60°C: about 45% of maximal activity, 75°C: about 75% of maximal activity, 80°C: about 10% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 65
-
 half-life: 13 h Bacillus sp. (in: Bacteria)
4.2.2.2 70
-
 half-life: 1 h Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 7 8 pH 7.0: about 35% of maximal activity, pH 8.0: optimum, pH 9.0: no activity Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.2 Bacillus sp. (in: Bacteria) isoelectric focusing
-
 5.3