Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Janosik, M.; Kery, V.; Gaustadnes, M.; Maclean, K.N.; Kraus, J.P.
    Regulation of human cystathionine beta-synthase by S-adenosyl-L-methionine: evidence for two catalytically active conformations involving an autoinhibitory domain in the C-terminal region (2001), Biochemistry, 40, 10625-10633.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.22 S-adenosyl-L-methionine induces conformational change Homo sapiens

Application

EC Number Application Comment Organism
4.2.1.22 medicine inherited deficiency leads to homocystinuria, an autosomal recessivlely inherited disease of sulfur metabolism Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.22
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.22 I435T inducible by S-adenosyl-L-methionine, but less responsive than wild-type enzyme to physiologically relevant concentrations Homo sapiens
4.2.1.22 S466L not inducible by S-adenosyl-L-methionine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 0.05
-
 homocysteine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 0.08
-
 homocysteine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 0.1
-
 homocysteine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 0.17
-
 homocysteine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 0.2
-
 homocysteine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 3.6
-
 L-serine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 4.2
-
 L-serine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 4.5
-
 L-serine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 4.9
-
 L-serine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 7.2
-
 L-serine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.22 486000
-
 octamer, gel filtration Homo sapiens
4.2.1.22 1330000
-
 multimer, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.22 two patient-derived forms S466L and I435T, 95% purity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-Serine + homocysteine
-
 Homo sapiens Cystathionine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.22 multimer wild-type enzyme and I435T mutant, gel filtration Homo sapiens
4.2.1.22 octamer wild-type enzyme and I435T mutant, gel filtration Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.22 55
-
 melting point of wild type and S466L mutant Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.22 5.4
-
 L-serine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 5.9
-
 L-serine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 5.9
-
 homocysteine I435T mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 6.2
-
 homocysteine wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 14.6
-
 L-serine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 15.5
-
 homocysteine S466L mutant, 37°C, absence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 15.8
-
 L-serine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 19.7
-
 L-serine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 32.1
-
 homocysteine I435T mutant, 37°C, presence of S-adenosyl-L-methionine Homo sapiens
4.2.1.22 34
-
 homocysteine wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine Homo sapiens