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Literature summary extracted from

  • Iverson, T.M.; Alber, B.E.; Kisker, C.; Ferry, J.G.; Rees, D.C.
    A closer look at the active site of gamma-class carbonic anhydrases: High resolution crystallographic studies of the carbonic anhydrase from Methanosarcina thermophila (2000), Biochemistry, 39, 9222-9231.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 produced in Escherichia coli Methanosarcina thermophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.1 cubic crystals, space group P2(1)3, Zn-Cam, unit cell a : b : c: 82.64 A, Zn-Cam-HCO3-, unit cell a : b : c: 82.51 A, Zn-Cam-SO42-, unit cell a : b : c: 82.68 A, Co-Cam, unit cell a : b : c: 82.34 A, Co-Cam-HCO3-, unit cell a : b : c: 82.58 A, Co-Cam-SO42-, unit cell a : b : c: 82.61 A Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Co2+ can substitute for Zn2+ Methanosarcina thermophila
4.2.1.1 Zn2+ zinc-containing enzyme Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Methanosarcina thermophila
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanosarcina thermophila P40881
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant enzyme Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Methanosarcina thermophila CO2 + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 Cam
-
 Methanosarcina thermophila
4.2.1.1 gamma-class carbonic anhydrase
-
 Methanosarcina thermophila