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Literature summary extracted from

  • Avarbock, D.; Avarbock, A.; Rubin, H.
    Differential regulation of opposing RelMtb activities by the aminoacylation state of a tRNA ribosome mRNA elMtb complex (2000), Biochemistry, 39, 11640-11648.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.7.2 medicine RelMtb is a key factor in Mycobacterium tuberculosis pathogenesis by regulating th intracellular concentration of (p)ppGpp. Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.7.2 the pET22b expression system is used Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.7.2 0.41
-
 ppGpp pH 8.0, 30°C, KM is increased 3fold in the presence of the complex containing tRNA, ribosomes, and mRNA Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.7.2 Mn2+ required, the optimal concentration is a 2-3fold molar excess over the nucleotide substrate, the addition in excess of optimal concentration decreases the rate of hydrolysis Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.7.2 ppGpp + H2O Mycobacterium tuberculosis i.e. guanosine-3',5'-bis(diphosphate) Gpp + diphosphate i.e. guanosine 5'-diphosphate r

Organism

EC Number Organism UniProt Comment Textmining
3.1.7.2 Mycobacterium tuberculosis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.7.2 guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate RelMtb is a dual-function enzyme carrying out ATP: GTP/GDP/ITP 3’-diphosphoryltransferase and (p)ppGpp 3’-diphosphohydrolase reactions. The differential regulation of the opposing activities of RelMtb is dependent on the ratio of uncharged to charged tRNA and the association of enzyme with a complex containing tRNA, ribosomes, and mRNA, i.e. RAC Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.7.2 ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Mycobacterium tuberculosis Gpp + diphosphate i.e. guanosine 5'-diphosphate ?
3.1.7.2 ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Mycobacterium tuberculosis Gpp + diphosphate i.e. guanosine 5'-diphosphate r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.7.2 2.97
-
 ppGpp pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA Mycobacterium tuberculosis
3.1.7.2 3 6 ppGpp pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.7.2 8 8.2
-
 Mycobacterium tuberculosis