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Literature summary extracted from

  • Roudiak, S.G.; Seth, A.; Knipfer, N.; Shrader, T.E.
    The lon protease from Mycobacterium smegmatis: molecular cloning, sequence analysis, functional expression, and enzymatic characterization (1998), Biochemistry, 37, 377-386.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.53 additional information stimulated by unfolded protein and supported by nonhydrolyzed nucleotide analogs Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.53 cloned and expressed in Escherichia coli MC1061 Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.53 S675A constructed mutation of the active site region Mycolicibacterium smegmatis
3.4.21.53 S675C constructed mutation of the active site region Mycolicibacterium smegmatis
3.4.21.53 S675T constructed mutation of the active site region Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.53 87000
-
 4 * 87000, homotetramer Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.53 RcsA + H2O Escherichia coli protein degradation mediates the turnover of damaged proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Escherichia coli
-
-
-
3.4.21.53 Mycolicibacterium smegmatis
-
 ATCC 607
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.53
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.53 Gln-Ala-Ala-Phe-p-nitroanilide + H2O preferred substrate Mycolicibacterium smegmatis ?
-
 ?
3.4.21.53 additional information succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide is not cleaved Mycolicibacterium smegmatis ?
-
 ?
3.4.21.53 RcsA + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.53 RcsA + H2O
-
 Mycolicibacterium smegmatis ?
-
 ?
3.4.21.53 RcsA + H2O protein degradation mediates the turnover of damaged proteins Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.53 tetramer 4 * 87000, homotetramer Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.4.21.53 lon protease
-
 Escherichia coli
3.4.21.53 lon protease
-
 Mycolicibacterium smegmatis
3.4.21.53 Ms-Lon
-
 Mycolicibacterium smegmatis