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Literature summary extracted from

  • Price, L.J.; Herbert, D.; Moss, S.R.; Cole, D.J.; Harwood, J.L.
    Graminicide insensitivity correlates with herbicide-binding co-operativity on acetyl-CoA carboxylase isoforms (2003), Biochem. J., 375, 415-423.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 Fluazifop
-
 Alopecurus myosuroides
6.4.1.2 Fluazifop
-
 Festuca rubra
6.4.1.2 Fluazifop
-
 Zea mays
6.4.1.2 Quizalofop
-
 Alopecurus myosuroides
6.4.1.2 Quizalofop
-
 Festuca rubra
6.4.1.2 Quizalofop
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.01
-
 acetyl-CoA cytosolic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 0.08
-
 ATP cytosolic enzyme, pH 8.0, 30°C Zea mays
6.4.1.2 0.08
-
 ATP plastidic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 0.08
-
 ATP plastidic enzyme, pH 8.0, 30°C Zea mays
6.4.1.2 0.11
-
 acetyl-CoA cytosolic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 0.11
-
 acetyl-CoA cytosolic enzyme, pH 8.0, 30°C Zea mays
6.4.1.2 0.11
-
 acetyl-CoA cytosolic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 0.12
-
 ATP cytosolic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 0.12
-
 acetyl-CoA plastidic enzyme, pH 8.0, 30°C Zea mays
6.4.1.2 0.14
-
 acetyl-CoA plastidic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 0.15
-
 ATP plastidic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 0.16
-
 acetyl-CoA plastidic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 0.18
-
 ATP cytosolic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 0.2
-
 acetyl-CoA plastidic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 0.39
-
 HCO3- cytosolic enzyme, pH 8.0, 30°C Zea mays
6.4.1.2 0.47
-
 ATP plastidic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 0.5
-
 ATP cytosolic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 1.09
-
 HCO3- plastidic enzyme, pH 8.0, 30°C Zea mays
6.4.1.2 1.15
-
 HCO3- plastidic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 1.25
-
 HCO3- cytosolic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 1.38
-
 HCO3- cytosolic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 1.38
-
 HCO3- plastidic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 1.64
-
 HCO3- plastidic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 1.75
-
 HCO3- cytosolic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.4.1.2 chloroplast major isoform Zea mays 9507
-
6.4.1.2 chloroplast major isoform Alopecurus myosuroides 9507
-
6.4.1.2 chloroplast major isoform Festuca rubra 9507
-
6.4.1.2 cytoplasm minor isoform Zea mays 5737
-
6.4.1.2 cytoplasm minor isoform Alopecurus myosuroides 5737
-
6.4.1.2 cytoplasm minor isoform Festuca rubra 5737
-
6.4.1.2 plastid
-
 Zea mays 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 219000
-
 SDS-PAGE, cytosolic isoform Zea mays
6.4.1.2 227000
-
 SDS-PAGE, plastidic isoform Zea mays
6.4.1.2 240000
-
 SDS-PAGE, both isoforms Festuca rubra

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Zea mays key enzyme of fatty acid biosynthesis ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Alopecurus myosuroides key enzyme of fatty acid biosynthesis ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- Festuca rubra key enzyme of fatty acid biosynthesis ADP + phosphate + malonyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Alopecurus myosuroides
-
 'Notts-A1'-resistant biotype and strain Rothamsted
-
6.4.1.2 Festuca rubra
-
-
-
6.4.1.2 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2 separation of two isoforms Zea mays
6.4.1.2 separation of two isoforms Alopecurus myosuroides
6.4.1.2 separation of two isoforms Festuca rubra

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 3.5
-
 purified cytosolic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 24.9
-
 purified cytosolic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 34.9
-
 purified cytosolic enzyme, pH 8.0, 30°C Zea mays
6.4.1.2 41.3
-
 purified cytosolic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 52.4
-
 purified plastidic enzyme from strain Notts A1, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 154.9
-
 purified plastidic enzyme from strain Rothamsted, pH 8.0, 30°C Alopecurus myosuroides
6.4.1.2 184.2
-
 purified plastidic enzyme, pH 8.0, 30°C Festuca rubra
6.4.1.2 217
-
 purified plastidic enzyme, pH 8.0, 30°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Zea mays ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Alopecurus myosuroides ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Festuca rubra ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- key enzyme of fatty acid biosynthesis Zea mays ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- key enzyme of fatty acid biosynthesis Alopecurus myosuroides ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- key enzyme of fatty acid biosynthesis Festuca rubra ADP + phosphate + malonyl-CoA
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 biotin enzyme-bound Zea mays
6.4.1.2 biotin enzyme-bound Alopecurus myosuroides
6.4.1.2 biotin enzyme-bound Festuca rubra