EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.78 | expression in Escherichia coli, His6-tag | Crithidia fasciculata |
6.3.1.8 | expression of a recombinant histidine-tagged enzyme in Escherichia coli | Crithidia fasciculata |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.78 | C79A | complete loss of amidase activity without affecting synthetase activity | Crithidia fasciculata |
6.3.1.8 | C79A | mutant retains synthetase activity, but loses amidase activity | Crithidia fasciculata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.78 | 0.059 | - |
spermidine | pH 7.3, synthetase activity | Crithidia fasciculata | |
3.5.1.78 | 0.114 | - |
ATP | pH 7.3, synthetase activity | Crithidia fasciculata | |
3.5.1.78 | 0.242 | - |
reduced glutathione | pH 7.3, synthetase activity | Crithidia fasciculata | |
3.5.1.78 | 0.5 | - |
glutathionylspermidine | pH 7.3, amidase activity | Crithidia fasciculata | |
6.3.1.8 | 0.059 | - |
spermidine | pH 7.3, 25°C, synthetase activity | Crithidia fasciculata | |
6.3.1.8 | 0.078 | - |
spermidine | pH 7.3, 25°C, synthetase activity of the C79A mutant | Crithidia fasciculata | |
6.3.1.8 | 0.096 | - |
MgADP- | pH 7.3, 25°C, synthetase activity of the C79A mutant | Crithidia fasciculata | |
6.3.1.8 | 0.114 | - |
MgADP- | pH 7.3, 25°C, synthetase activity | Crithidia fasciculata | |
6.3.1.8 | 0.171 | - |
glutathione | pH 7.3, 25°C, synthetase activity of the C79A mutant | Crithidia fasciculata | |
6.3.1.8 | 0.242 | - |
glutathione | pH 7.3, 25°C, synthetase activity | Crithidia fasciculata | |
6.3.1.8 | 0.5 | - |
glutathionylspermidine | pH 7.3, 25°C, amidase activity | Crithidia fasciculata |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.1.8 | 80520 | - |
MALDI-TOF MS | Crithidia fasciculata |
6.3.1.8 | 85200 | - |
gel filtration | Crithidia fasciculata |
6.3.1.8 | 86000 | - |
1 * 86000, SDS-PAGE | Crithidia fasciculata |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.78 | Crithidia fasciculata | - |
bifunctional glutathionylspermidine synthetase, EC 6.3.1.8, and amidase, EC 3.5.1.78 | - |
6.3.1.8 | Crithidia fasciculata | P90518 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.78 | recombinant enzyme | Crithidia fasciculata |
6.3.1.8 | using Ni-chelating and anion-exchange chromatography | Crithidia fasciculata |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.8 | 7.9 | - |
pH 7.3, 25°C, synthetase activity | Crithidia fasciculata |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.1.8 | -80°C, 4 months, loses less than 10% synthetase activity | Crithidia fasciculata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.78 | glutathionylspermidine + H2O | - |
Crithidia fasciculata | glutathione + spermidine | - |
r | |
3.5.1.78 | trypanothione + H2O | hydrolysis at 1.5% the rate of glutathionylspermidine | Crithidia fasciculata | glutathione + glutathionylspermidine | - |
? | |
6.3.1.8 | gamma-L-Glu-L-Cys-Gly + spermidine + MgATP2- | - |
Crithidia fasciculata | N1-(gamma-L-Glu-L-Cys-Gly)-spermidine + MgADP- + phosphate | - |
? | |
6.3.1.8 | glutathionylspermidine + H2O | amidase activity | Crithidia fasciculata | glutathione + spermidine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.1.8 | monomer | 1 * 86000, SDS-PAGE | Crithidia fasciculata |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.8 | GspS | - |
Crithidia fasciculata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.8 | 7.31 | - |
- |
Crithidia fasciculata |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.8 | 6.8 | 8 | - |
Crithidia fasciculata |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
6.3.1.8 | Crithidia fasciculata | isoelectric focusing | 4.7 | 4.6 |