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Literature summary extracted from

  • Bae, S.H.; Seong, J.; Paik, Y.K.
    Cholesterol biosynthesis from lanosterol: molecular cloning, chromosomal localization, functional expression and liver-specific gene regulation of rat sterol DELTA8-isomerase, a cholesterogenic enzyme with multiple functions (2001), Biochem. J., 353, 689-699.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.5 expression in Saccharomyces cerevisiae Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.5 3beta-[2-(diethylamino)ethoxy]androst-5-en-17-one IC50: 0.0042 mM Rattus norvegicus
5.3.3.5 trans-2-[4-(1,2-diphenylbuten-1-yl)phenoxy]-N,N-dimethylethylamine IC50: 0.0112 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.5 26737
-
 x * 26737, calculation from nucleotide sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.5 5alpha-cholest-7-en-3beta-ol Rattus norvegicus enzyme of the cholesterol biosynthetic pathway, mutations are associated with Conradi-Huenermann syndrome. The enzyme may be involved in liver function, since the mRNA is only detected in hepatocytes not in connective tissue or blood vessels. The expression of the SI gene is regulated transcriptionally in vitro and in vivo by widely known regulatory agents ranging from tamoxifen, an oestrogen receptor antagonist and SI-specific inhibitor, to cholesterol-lowering drugs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.5 Rattus norvegicus Q9JJ46
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.5 5alpha-cholest-7-en-3beta-ol enzyme of the cholesterol biosynthetic pathway, mutations are associated with Conradi-Huenermann syndrome. The enzyme may be involved in liver function, since the mRNA is only detected in hepatocytes not in connective tissue or blood vessels. The expression of the SI gene is regulated transcriptionally in vitro and in vivo by widely known regulatory agents ranging from tamoxifen, an oestrogen receptor antagonist and SI-specific inhibitor, to cholesterol-lowering drugs Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.3.5 ? x * 26737, calculation from nucleotide sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
5.3.3.5 SI
-
 Rattus norvegicus
5.3.3.5 sterol DELTA8 isomerase
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.3.3.5 0.0042
-
 IC50: 0.0042 mM Rattus norvegicus 3beta-[2-(diethylamino)ethoxy]androst-5-en-17-one
5.3.3.5 0.0112
-
 IC50: 0.0112 mM Rattus norvegicus trans-2-[4-(1,2-diphenylbuten-1-yl)phenoxy]-N,N-dimethylethylamine